Bacterial mandelic acid degradation pathway and its application in biotechnology
Q Wang, S Geng, L Wang, Z Wen… - Journal of Applied …, 2022 - academic.oup.com
Mandelic acid and its derivatives are an important class of chemical synthetic blocks, which
is widely used in drug synthesis and stereochemistry research. In nature, mandelic acid …
is widely used in drug synthesis and stereochemistry research. In nature, mandelic acid …
A paradigm for CH bond cleavage: structural and functional aspects of transition state stabilization by mandelate racemase
SL Bearne, MS Maurice - Advances in protein chemistry and structural …, 2017 - Elsevier
Mandelate racemase (MR) from Pseudomonas putida catalyzes the Mg 2+-dependent, 1, 1-
proton transfer reaction that racemizes (R)-and (S)-mandelate. MR shares a partial reaction …
proton transfer reaction that racemizes (R)-and (S)-mandelate. MR shares a partial reaction …
Polymersomes as nanoreactors enabling the application of solvent‐sensitive enzymes in different biphasic reaction setups
F Golombek, K Castiglione - Biotechnology Journal, 2020 - Wiley Online Library
There is an increasing interest in biocatalysis to perform chemical reactions in biphasic
systems, consisting of an aqueous phase and a water‐immiscible organic solvent or ionic …
systems, consisting of an aqueous phase and a water‐immiscible organic solvent or ionic …
The impact of a His-tag on DNA binding by RNA polymerase alpha-C-terminal domain from Helicobacter pylori
Polyhistidine tags (His-tags) are commonly employed in protein purification strategies due to
the high affinity and specificity for metal-NTA columns, the relative simplicity of such …
the high affinity and specificity for metal-NTA columns, the relative simplicity of such …
Slow-onset, potent inhibition of mandelate racemase by 2-formylphenylboronic acid. An unexpected adduct clasps the catalytic machinery
CD Douglas, L Grandinetti, NM Easton, OP Kuehm… - Biochemistry, 2021 - ACS Publications
o-Carbonyl arylboronic acids such as 2-formylphenylboronic acid (2-FPBA) are employed in
biocompatible conjugation reactions with the resulting iminoboronate adduct stabilized by …
biocompatible conjugation reactions with the resulting iminoboronate adduct stabilized by …
Structure of mandelate racemase with bound intermediate analogues benzohydroxamate and cupferron
AD Lietzan, M Nagar, EA Pellmann, JR Bourque… - Biochemistry, 2012 - ACS Publications
Mandelate racemase (MR, EC 5.1. 2.2) from Pseudomonas putida catalyzes the Mg2+-
dependent interconversion of the enantiomers of mandelate, stabilizing the altered substrate …
dependent interconversion of the enantiomers of mandelate, stabilizing the altered substrate …
Potent inhibition of mandelate racemase by a fluorinated substrate-product analogue with a novel binding mode
Mandelate racemase (MR) from Pseudomonas putida catalyzes the Mg2+-dependent 1, 1-
proton transfer that interconverts the enantiomers of mandelate. Because trifluorolactate is …
proton transfer that interconverts the enantiomers of mandelate. Because trifluorolactate is …
Application of circular dichroism-based assays to racemases and epimerases: Recognition and catalysis of reactions of chiral substrates by mandelate racemase
SL Bearne, JA Hayden - Methods in Enzymology, 2023 - Elsevier
Racemases and epimerases have attracted much interest because of their astonishing
ability to catalyze the rapid α-deprotonation of carbon acid substrates with high p K a values …
ability to catalyze the rapid α-deprotonation of carbon acid substrates with high p K a values …
Three steps, two enzymes, one pot, but a multitude of nanocompartments: Combined cycles of kinetic resolutions and re-racemization with incompatible biocatalysts
F Golombek, M Haumann, MSG Knoll, AP Froba… - ACS …, 2021 - ACS Publications
Deracemizations are clearly preferable to kinetic resolutions in the production of chiral
molecules from racemates, as they allow up to 100% chemical and optical yield. Here we …
molecules from racemates, as they allow up to 100% chemical and optical yield. Here we …
Structure-guided design of an engineered streptavidin with reusability to purify streptavidin-binding peptide tagged proteins or biotinylated proteins
SC Wu, SL Wong - PloS one, 2013 - journals.plos.org
Development of a high-affinity streptavidin-binding peptide (SBP) tag allows the tagged
recombinant proteins to be affinity purified using the streptavidin matrix without the need of …
recombinant proteins to be affinity purified using the streptavidin matrix without the need of …