Biocatalysis has become an important aspect of modern organic synthesis, both in
academia and across the chemical and pharmaceutical industries. Its success has been …

Biocatalysis has undergone revolutionary progress in the past century. Benefited by the
integration of multidisciplinary technologies, natural enzymatic reactions are constantly …

Directed evolution of stereo‐, regio‐, and chemoselective enzymes constitutes a unique way
to generate biocatalysts for synthetically interesting transformations in organic chemistry and …

Enzyme turnover numbers (k cat) are key to understanding cellular metabolism, proteome
allocation and physiological diversity, but experimentally measured k cat data are sparse …

Raman microspectroscopy offers microbiologists a rapid and non-destructive technique to
assess the chemical composition of individual live microorganisms in near real time. In this …

Activity-based protein profiling (ABPP) is a technique that uses highly selective active-site
targeted chemical probes to label and monitor the state of proteins. ABPP integrates the …

Nanozymes are nanomaterials with similar catalytic activities to natural enzymes. Compared
with natural enzymes, they have numerous advantages, including higher physiochemical …

The two main strategies for enzyme engineering, directed evolution and rational design,
have found widespread applications in improving the intrinsic activities of proteins. Although …

Enzymatic carbon dioxide fixation is one of the most important metabolic reactions as it
allows the capture of inorganic carbon from the atmosphere and its conversion into organic …

微生物工程菌株是生物制造的重要基础, 但大多数的工程菌株需要进化改造才能适用于生物制造
. 在菌种选育过程中, 如何高效地筛选获得具有目标性状的微生物工程菌株是进行生物制造应用 …