The biosynthesis of natural products by heterologous expression of biosynthetic pathways in
amenable production strains enables biotechnological access to a variety of valuable …

The cryptic secondary metabolite biosynthetic gene clusters (BGCs) far outnumber currently
known secondary metabolites. Heterologous production of secondary metabolite BGCs in …

The soil bacterium Pseudomonas putida is increasingly attracting considerable interest as a
platform for advanced metabolic engineering through synthetic biology approaches …

Background The microbial production of biofuels is complicated by a tradeoff between yield
and toxicity of many fuels. Efflux pumps enable bacteria to tolerate toxic substances by their …

Biocatalysis has become an essential tool in the chemical industry and is the core of
industrial biotechnology, also known as white biotechnology, making use of biocatalysts in …

Understanding the composability of genetic elements is central to synthetic biology. Even for
seemingly well-known elements such as a sigma 70 promoter the genetic context …

Pseudomonads are ubiquitous Gram-negative bacteria found in soil, water, air, food, plants,
and animals, some interacting with other organisms as facultative pathogens and …

Genomic integration is commonly used to engineer stable production hosts. However, so far,
for many microbial workhorses, only a few integration sites have been characterized …

Pseudomonas putida S12 is a solvent-tolerant gamma-proteobacterium with an extensive
track record for production of industrially relevant chemicals. Here we report the annotated …

Previously, an efficient d-xylose utilizing Pseudomonas putida S12 strain was obtained by
introducing the d-xylose isomerase pathway from Escherichia coli, followed by evolutionary …