Since the Human Genome Project completed in 2000, the sequencing of the first genome,
massive progress has been made by medical science in the early diagnosis and …

Compared to temperature, the development of pressure as a tool in the research field has
emerged only recently (at the end of the XIXth century). Following several developments in …

Abstract Phosphatidylinositol-3, 4, 5-trisphosphate (PtdIns (3, 4, 5) P3 or PIP3) mediates
signalling pathways as a second messenger in response to extracellular signals. Although …

BACKGROUND Effective upstream preparation of nucleic acid (NA) is important for
molecular techniques that detect unique DNA or RNA sequences. The isolated NA should …

Robust methodologies for the analysis of fecal material will facilitate the understanding of
gut (patho) physiology and its role in health and disease and will help improve care for …

Mass spectrometry (MS) has been proven as an excellent tool in ocular drug research
allowing analyzes from small samples and low concentrations. This review begins with a …

Liquid chromatography-tandem mass spectrometry (LC-MS/MS)-based proteomics provides
a wealth of information about proteins present in biological samples. In bottom-up LC …

We have developed a streamlined proteomic sample preparation protocol termed
Accelerated Barocycler Lysis and Extraction (ABLE) that substantially reduces the time and …

In biology, scientist's interest for high hydrostatic pressure (HHP) has increased over the last
20 years, for both research and industrial developments, mainly because of the low energy …

This study reports the development of a two-step protocol using pressure cycling technology
(PCT) and alkaline lysis for differential extraction of DNA from mixtures of sperm and vaginal …