The term B-factor, sometimes called the Debye–Waller factor, temperature factor, or atomic
displacement parameter, is used in protein crystallography to describe the attenuation of X …

Lipases and phospholipases are interfacial enzymes that hydrolyze hydrophobic ester
linkages of triacylglycerols and phospholipids, respectively. In addition to their role as …

Background Disulfide engineering is an important biotechnological tool that has advanced a
wide range of research. The introduction of novel disulfide bonds into proteins has been …

Enzymes found in nature have been exploited in industry due to their inherent catalytic
properties in complex chemical processes under mild experimental and environmental …

Improving the stability of proteins is an important goal in many biomedical and industrial
applications. A logical approach is to emulate stabilizing molecular interactions found in …

Enzyme stability is an important issue for protein engineers. Understanding how rigidity in
the active site affects protein kinetic stability will provide new insight into enzyme …

Flexible sites are potential targets for engineering the stability of enzymes. Nevertheless, the
success rate of the rigidifying flexible sites (RFS) strategy is still low due to a limited …

Engineering proteins for thermostability is an exciting and challenging field since it is critical
for broadening the industrial use of recombinant proteins. Thermostability of proteins arises …

Plastics are highly durable and widely used materials. Current methodologies of plastic
degradation, elimination, and recycling are flawed. In recent years, biodegradation (the …

Lipase catalyzes hydrolysis of fats in lipid water interphase and perform variety of
biotransformation reactions under micro aqueous conditions. The major sources include …