Preparation of isolated rat liver cells

PO Seglen - Methods in cell biology, 1976 - Elsevier
Publisher Summary This chapter discusses preparation of isolated rat liver cells. The early
mechanical and chemical methods for liver-cell preparation were relatively successful in …

Scavenger functions of the liver endothelial cell.

B Smedsrød, H Pertoft, S Gustafson… - Biochemical …, 1990 - ncbi.nlm.nih.gov
MORPHOLOGY AND CHARACTERIZATION Prior to Aterman's and Wisse's important
electron microscopic studies (Aterman, 1963; Wisse, 1970, 1972), very little was known …

Distribution and tissue uptake of poly (ethylene glycol) with different molecular weights after intravenous administration to mice

T Yamaoka, Y Tabata, Y Ikada - Journal of pharmaceutical sciences, 1994 - Elsevier
After intravenous (iv) injection of 125 I-labeled poly (ethylene glycol)(PEG) with different
molecular weights to mice, the radioactivity of the organs was measured to …

An efficient method to isolate and culture mouse Kupffer cells

P Li, J Li, M Li, J Gong, K He - Immunology letters, 2014 - Elsevier
Kupffer cells (KCs) play an essential role in the physiological and pathological functions of
the liver. Although the isolation methods of KCs have been well-described, most of them are …

Endothelial cells are a site of uptake and degradation of hyaluronic acid in the liver

S Eriksson, JRE Fraser, TC Laurent, H Pertoft… - Experimental cell …, 1983 - Elsevier
In a recent communication it was shown that intravenously injected radioactively labelled
hyaluronic acid was preferentially taken up by the liver and degraded. We now report that …

[PDF][PDF] Malaria sporozoites actively enter and pass through rat Kupffer cells prior to hepatocyte invasion

G Pradel, U Frevert - Hepatology, 2001 - Wiley Online Library
Malaria sporozoites have to cross the layer of sinusoidal liver cells to reach their initial site of
multiplication in the mammalian host, the hepatocytes. To determine the sinusoidal cell type …

Plasma clearance of intravenously injected aspartate aminotransferase isozymes: evidence for preferential uptake by sinusoidal liver cells

Y Kamimoto, S Horiuchi, S Tanase, Y Morino - Hepatology, 1985 - journals.lww.com
Both cytosolic (c–AAT) and mitochondrial (m–AAT) isozymes of aspartate aminotransferase
(EC 2.6. 1.1) appear in serum in some diseases including hepatobiliary dysfunction. The …

The origin, kinetics, and characteristics of the Kupffer cells in the normal steady state

RW Crofton, MMC Diesselhoff-den Dulk… - The Journal of …, 1978 - rupress.org
Enzymatic digestion with pronase and DNAase was used to isolate Kupffer cells from mouse
liver. The characteristics of these cells were found to be similar to those of peritoneal …

[PDF][PDF] Recent advances in the isolation of liver cells

G Alpini, JO Phillips, B Vroman, NF Larusso - Hepatology, 1994 - Wiley Online Library
The liver consists of two types of epithelial cells: hepatocytes, which represent 60% to 70%
of the liver cell population (l), or 90% of total liver mass (2); and intrahepatic bile duct …

Separation of Kupffer and endothelial cells of the rat liver by centrifugal elutriation

DL Knook, EC Sleyster - Experimental cell research, 1976 - Elsevier
Isolated non-parenchymal cells from rat liver were separated by centrifugal elutriation into
two fractions consisting of structurally intact Kupffer and endothelial cells with purities of 91 …