Environmental DNA metabarcoding: Transforming how we survey animal and plant communities
The genomic revolution has fundamentally changed how we survey biodiversity on earth.
High‐throughput sequencing (“HTS”) platforms now enable the rapid sequencing of DNA …
High‐throughput sequencing (“HTS”) platforms now enable the rapid sequencing of DNA …
The ecology of environmental DNA and implications for conservation genetics
MA Barnes, CR Turner - Conservation genetics, 2016 - Springer
Environmental DNA (eDNA) refers to the genetic material that can be extracted from bulk
environmental samples such as soil, water, and even air. The rapidly expanding study of …
environmental samples such as soil, water, and even air. The rapidly expanding study of …
[HTML][HTML] Airborne environmental DNA for terrestrial vertebrate community monitoring
Biodiversity monitoring at the community scale is a critical element of assessing and
studying species distributions, ecology, diversity, and movements, and it is key to …
studying species distributions, ecology, diversity, and movements, and it is key to …
Counting with DNA in metabarcoding studies: How should we convert sequence reads to dietary data?
Advances in DNA sequencing technology have revolutionized the field of molecular analysis
of trophic interactions, and it is now possible to recover counts of food DNA sequences from …
of trophic interactions, and it is now possible to recover counts of food DNA sequences from …
DNA metabarcoding—Need for robust experimental designs to draw sound ecological conclusions
DNA metabarcoding, especially when coupled with high‐through‐put DNA sequencing, is
currently revolutionizing our capacity to assess biodiversity across a full range of taxa and …
currently revolutionizing our capacity to assess biodiversity across a full range of taxa and …
Scrutinizing key steps for reliable metabarcoding of environmental samples
Metabarcoding of environmental samples has many challenges and limitations that require
carefully considered laboratory and analysis workflows to ensure reliable results. We …
carefully considered laboratory and analysis workflows to ensure reliable results. We …
Ecosystem biomonitoring with eDNA: metabarcoding across the tree of life in a tropical marine environment
Effective marine management requires comprehensive data on the status of marine
biodiversity. However, efficient methods that can document biodiversity in our oceans are …
biodiversity. However, efficient methods that can document biodiversity in our oceans are …
[图书][B] A practical guide to DNA-based methods for biodiversity assessment.
This book represents a synthesis of knowledge and best practice in the field of DNA-based
biomonitoring at the time of writing. It has been written with end-users of molecular tools in …
biomonitoring at the time of writing. It has been written with end-users of molecular tools in …
PCR inhibition in qPCR, dPCR and MPS—mechanisms and solutions
DNA analysis has seen an incredible development in terms of instrumentation, assays and
applications over the last years. Massively parallel sequencing (MPS) and digital PCR are …
applications over the last years. Massively parallel sequencing (MPS) and digital PCR are …
An assessment of minimum sequence copy thresholds for identifying and reducing the prevalence of artefacts in dietary metabarcoding data
LE Drake, JP Cuff, RE Young… - Methods in Ecology …, 2022 - Wiley Online Library
Metabarcoding provides a powerful tool for investigating biodiversity and trophic
interactions, but the high sensitivity of this methodology makes it vulnerable to errors …
interactions, but the high sensitivity of this methodology makes it vulnerable to errors …