Background Spermatogenesis is a complex differentiation process that involves the
successive and simultaneous execution of three different gene expression programs: mitotic …

Background: This review aims to address procedures and indications for the application of
the adipose-derived stem cells (ADSCs) for regenerative dentistry. ADSCs have rarely been …

Mammalian spermatogenesis is nowadays still poorly understood at the molecular level,
mainly due to the heterogeneous nature of testes, which contain a high number of different …

Protocols for purification of murine male germ cells by FACS based on Hoechst 33342
(Ho342) dye staining have been reported and optimized. However, the protocols are often …

More than 50% of cases of primary ovarian insufficiency (POI) and nonobstructive
azoospermia in humans are classified as idiopathic infertility. Meiotic defects may relate to at …

Mammalian spermatogenesis is a complex process that involves spatiotemporal regulation
of gene expression and meiotic recombination, both of which require the modulation of …

Testis-specific genes are prone to affect spermatogenesis or sperm fertility, and thus may
play pivotal roles in male reproduction. However, whether a gene really affects male …

Objective To compare mechanical dissociation, employing the Medimachine system, and
enzymatic digestion of human testicular tissues with respect to the proportion of …

Mammalian testes are very complex organs that contain over 30 different cell types,
including somatic testicular cells and different stages of germline cells. This heterogeneity is …

Fluorescence-activated cell sorting (FACS) has been one of the methods of choice to isolate
enriched populations of mammalian testicular germ cells. Currently, it allows the …