Cellular Site-Specific Incorporation of Noncanonical Amino Acids in Synthetic Biology
W Niu, J Guo - Chemical Reviews, 2024 - ACS Publications
Over the past two decades, genetic code expansion (GCE)-enabled methods for
incorporating noncanonical amino acids (ncAAs) into proteins have significantly advanced …
incorporating noncanonical amino acids (ncAAs) into proteins have significantly advanced …
Using genetically incorporated unnatural amino acids to control protein functions in mammalian cells
AR Nödling, LA Spear, TL Williams… - Essays in …, 2019 - portlandpress.com
Genetic code expansion allows unnatural (non-canonical) amino acid incorporation into
proteins of interest by repurposing the cellular translation machinery. The development of …
proteins of interest by repurposing the cellular translation machinery. The development of …
Time-resolved protein activation by proximal decaging in living systems
A universal gain-of-function approach for selective and temporal control of protein activity in
living systems is crucial to understanding dynamic cellular processes. Here we report …
living systems is crucial to understanding dynamic cellular processes. Here we report …
Dissociative bioorthogonal reactions
Bioorthogonal reactions that proceed readily under physiological conditions without
interference from biomolecules have found widespread application in the life sciences …
interference from biomolecules have found widespread application in the life sciences …
Recent technologies for genetic code expansion and their implications on synthetic biology applications
Genetic code expansion (GCE) enables the site-specific incorporation of non-canonical
amino acids as novel building blocks for the investigation and manipulation of proteins. The …
amino acids as novel building blocks for the investigation and manipulation of proteins. The …
Chemogenetic and optogenetic control of post-translational modifications through genetic code expansion
Post-translational modifications (PTMs) of proteins extensively diversify the biological
information flow from the genome to the proteome and thus have profound …
information flow from the genome to the proteome and thus have profound …
Artificial Small Molecules as Cofactors and Biomacromolecular Building Blocks in Synthetic Biology: Design, Synthesis, Applications, and Challenges
F Liu, L He, S Dong, J Xuan, Q Cui, Y Feng - Molecules, 2023 - mdpi.com
Enzymes are essential catalysts for various chemical reactions in biological systems and
often rely on metal ions or cofactors to stabilize their structure or perform functions …
often rely on metal ions or cofactors to stabilize their structure or perform functions …
Bioorthogonal Engineering of Bacterial Effectors for Spatial–Temporal Modulation of Cell Signaling
The complicated and entangled cell signaling network is dynamically regulated by a wide
array of enzymes such as kinases. It remains desirable but challenging to specifically …
array of enzymes such as kinases. It remains desirable but challenging to specifically …
Expansion of the genetic code
The genetic code consists of codons that code for 20 canonical amino acids and 3
termination signals and is an indispensable element of life. It controls all cellular activities …
termination signals and is an indispensable element of life. It controls all cellular activities …
Optical Control of CRISPR/Cas9 and Kinase Function
W Zhou - 2022 - d-scholarship.pitt.edu
Genetic code expansion provides a unique solution by allowing for site-specific installation
of functionally masked unnatural amino acids (UAAs) into proteins, rendering them inert until …
of functionally masked unnatural amino acids (UAAs) into proteins, rendering them inert until …