Is enzyme immobilization a mature discipline? Some critical considerations to capitalize on the benefits of immobilization

JM Bolivar, JM Woodley… - Chemical Society …, 2022 - pubs.rsc.org
Enzyme immobilization has been developing since the 1960s and although many industrial
biocatalytic processes use the technology to improve enzyme performance, still today we …

[HTML][HTML] Stabilization of enzymes via immobilization: Multipoint covalent attachment and other stabilization strategies

RC Rodrigues, Á Berenguer-Murcia… - Biotechnology …, 2021 - Elsevier
The use of enzymes in industrial processes requires the improvement of their features in
many instances. Enzyme immobilization, a requirement to facilitate the recovery and reuse …

Parameters necessary to define an immobilized enzyme preparation

J Boudrant, JM Woodley, R Fernandez-Lafuente - Process Biochemistry, 2020 - Elsevier
Biocatalytic processes continue to find increasing application in industry. Therefore enzyme
immobilization has also become of increasing importance as a means of allowing enzyme …

[HTML][HTML] Oriented immobilization of antibodies onto sensing platforms-A critical review

S Gao, JM Guisán, J Rocha-Martin - Analytica chimica acta, 2022 - Elsevier
The immunosensor has been proven a versatile tool to detect various analytes, such as food
contaminants, pathogenic bacteria, antibiotics and biomarkers related to cancer. To fabricate …

Current status and future perspectives of supports and protocols for enzyme immobilization

FTT Cavalcante, ALG Cavalcante, IG de Sousa… - Catalysts, 2021 - mdpi.com
The market for industrial enzymes has witnessed constant growth, which is currently around
7% a year, projected to reach $10.5 billion in 2024. Lipases are hydrolase enzymes …

On the taught new tricks of enzymes immobilization: An all-inclusive overview

RA Wahab, N Elias, F Abdullah, SK Ghoshal - Reactive and Functional …, 2020 - Elsevier
The primary means of immobilizing enzymes are to boost the enzyme productivity and
operational stability, alongside facilitating the reuse of enzymes. Notwithstanding the …

Importance of the support properties for immobilization or purification of enzymes

JCS Santos, O Barbosa, C Ortiz… - …, 2015 - Wiley Online Library
Immobilization and purification of enzymes are usual requirements for their industrial use.
Both purification and immobilization have a common factor: they use a solid activated …

Multi-point enzyme immobilization, surface chemistry, and novel platforms: a paradigm shift in biocatalyst design

M Bilal, M Asgher, H Cheng, Y Yan… - Critical reviews in …, 2019 - Taylor & Francis
Engineering enzymes with improved catalytic properties in non-natural environments have
been concerned with their diverse industrial and biotechnological applications …

Glutaraldehyde in bio-catalysts design: A useful crosslinker and a versatile tool in enzyme immobilization

O Barbosa, C Ortiz, Á Berenguer-Murcia, R Torres… - Rsc Advances, 2014 - pubs.rsc.org
Glutaraldehyde is one of the most widely used reagents in the design of biocatalysts. It is a
powerful crosslinker, able to react with itself, with the advantages that this may bring forth. In …

Potential of different enzyme immobilization strategies to improve enzyme performance

C Garcia‐Galan, Á Berenguer‐Murcia… - Advanced Synthesis …, 2011 - Wiley Online Library
Enzyme biocatalysis plays a very relevant role in the development of many chemical
industries, eg, energy, food or fine chemistry. To achieve this goal, enzyme immobilization is …