Metabolic co-dependence of the oocyte and cumulus cells: essential role in determining oocyte developmental competence

D Richani, KR Dunning, JG Thompson… - Human reproduction …, 2021 - academic.oup.com
BACKGROUND Within the antral follicle, the oocyte is reliant on metabolic support from its
surrounding somatic cells. Metabolism plays a critical role in oocyte developmental …

Noninvasive metabolic profiling of cumulus cells, oocytes, and embryos via fluorescence lifetime imaging microscopy: a mini-review

M Venturas, X Yang, D Sakkas… - Human …, 2023 - academic.oup.com
A major challenge in ART is to select high-quality oocytes and embryos. The metabolism of
oocytes and embryos has long been linked to their viability, suggesting the potential utility of …

Incubator type affects human blastocyst formation and embryo metabolism: a randomized controlled trial

AJ Kermack, I Fesenko, DR Christensen… - Human …, 2022 - academic.oup.com
STUDY QUESTION Does the type of incubator used to culture human preimplantation
embryos affect development to the blastocyst stage and alter amino acid utilization of …

[HTML][HTML] Embryo competence and cryosurvival: Molecular and cellular features

TV Marsico, J Camargo, RS Valente… - Animal Reproduction, 2019 - SciELO Brasil
Global cattle genetic market is experiencing a change of strategy, large genetic companies,
traditionally recognized in the artificial insemination field, have also begun to operate in the …

Non-invasive, label-free optical analysis to detect aneuploidy within the inner cell mass of the preimplantation embryo

TCY Tan, SB Mahbub, JM Campbell… - Human …, 2022 - academic.oup.com
STUDY QUESTION Can label-free, non-invasive optical imaging by hyperspectral
autofluorescence microscopy discern between euploid and aneuploid cells within the inner …

[HTML][HTML] Methods for assessing the quality of mammalian embryos: How far we are from the gold standard?

JC Rocha, F Passalia, FD Matos… - JBRA assisted …, 2016 - ncbi.nlm.nih.gov
Morphological embryo classification is of great importance for many laboratory techniques,
from basic research to the ones applied to assisted reproductive technology. However, the …

[HTML][HTML] The mitochondrial respiration signature of the bovine blastocyst reflects both environmental conditions of development as well as embryo quality

J Kurzella, D Miskel, F Rings, E Tholen, D Tesfaye… - Scientific Reports, 2023 - nature.com
The major limitation of the widespread use of IVP derived embryos is their consistent
deficiencies in vitality when compared with their ex vivo derived counterparts. Although …

UVA hyperspectral light-sheet microscopy for volumetric metabolic imaging: application to preimplantation embryo development

J Morizet, D Chow, P Wijesinghe, E Schartner… - ACS …, 2023 - ACS Publications
Cellular metabolism is a key regulator of energetics, cell growth, regeneration, and
homeostasis. Spatially mapping the heterogeneity of cellular metabolic activity is of great …

[HTML][HTML] Vitrification within a nanoliter volume: oocyte and embryo cryopreservation within a 3D photopolymerized device

SH Yagoub, M Lim, TCY Tan, DJX Chow… - Journal of Assisted …, 2022 - Springer
Purpose Vitrification permits long-term banking of oocytes and embryos. It is a technically
challenging procedure requiring direct handling and movement of cells between potentially …

[HTML][HTML] Multispectral autofluorescence characteristics of reproductive aging in old and young mouse oocytes

JM Campbell, SB Mahbub, MJ Bertoldo, A Habibalahi… - Biogerontology, 2022 - Springer
Increasing age has a major detrimental impact on female fertility, which, with an ageing
population, has major sociological implications. This impact is primarily mediated through …