Can dynamic in vitro digestion systems mimic the physiological reality?

D Dupont, M Alric, S Blanquet-Diot… - Critical reviews in …, 2019 - Taylor & Francis
During the last decade, there has been a growing interest in understanding the fate of food
during digestion in the gastrointestinal tract in order to strengthen the possible effects of food …

Microfluidics and microbial engineering

S Kou, D Cheng, F Sun, IM Hsing - Lab on a Chip, 2016 - pubs.rsc.org
The combination of microbial engineering and microfluidics is synergistic in nature. For
example, microfluidics is benefiting from the outcome of microbial engineering and many …

Proteome characterization of extracellular vesicles from human milk: Uncovering the surfaceome by a lipid‐based protein immobilization technology

E Ahlberg, MC Jenmalm, A Karlsson… - Journal of …, 2024 - Wiley Online Library
Breast milk is an essential source of nutrition and hydration for the infant. In addition, this
highly complex fluid is rich in extracellular vesicles (EVs). Here, we have applied a …

Identification of surface proteins in a clinical Staphylococcus haemolyticus isolate by bacterial surface shaving

R Wolden, M Pain, R Karlsson, A Karlsson… - BMC microbiology, 2020 - Springer
Abstract Background The skin commensal Staphylococcus haemolyticus is an emerging
nosocomial pathogen. Despite its clinical relevance, published information about S …

Rational antibody design for undruggable targets using kinetically controlled biomolecular probes

CL Trkulja, O Jungholm, M Davidson, K Jardemark… - Science …, 2021 - science.org
Several important drug targets, eg, ion channels and G protein–coupled receptors, are
extremely difficult to approach with current antibody technologies. To address these targets …

Subcellular localization of an ATPase in anammox bacteria using proteomics and immunogold electron microscopy

R Karlsson, A Karlsson, O Bäckman… - FEMS microbiology …, 2014 - academic.oup.com
Anaerobic ammonium oxidation (anammox) has received significant attention during
optimization of waste-water treatment and constitutes an important pathway for the removal …

A new sample preparation method for the absolute quantitation of a target proteome using 18 O labeling combined with multiple reaction monitoring mass …

J Li, L Zhou, H Wang, H Yan, N Li, R Zhai, F Jiao… - Analyst, 2015 - pubs.rsc.org
A key step in the workflow of bottom-up proteomics is the proteolysis of proteins into
peptides with trypsin. In addition, enzyme-catalytic 18O labeled peptides as internal …

Probing structure and function of ion channels using limited proteolysis and microfluidics

CL Trkulja, ET Jansson, K Jardemark… - Journal of the American …, 2014 - ACS Publications
Even though gain, loss, or modulation of ion channel function is implicated in many
diseases, both rare and common, the development of new pharmaceuticals targeting this …

An ultra-fast and highly efficient multiple proteases digestion strategy using graphene-oxide-based immobilized protease reagents

HH Bai, YT Pan, XJ Ren, FR Hao, SS Deng… - Science China …, 2014 - Springer
Highly efficient and rapid proteolytic digestion of proteins into peptides is a crucial step in
shotgun-based proteome-analysis strategy. Tandem digestion by two or more proteases is …

Activation and signaling characteristics of the hydroxy-carboxylic acid 3 receptor identified in human neutrophils through a microfluidic flow cell technique

H Forsman, W Li, NK Levin, R Karlsson, A Karlsson… - bioRxiv, 2024 - biorxiv.org
Human neutrophils express numerous G protein-coupled receptors (GPCRs) of importance
for immune regulation. Several of the functionally known neutrophil GPCRs, are not part of …