Advances in microscopy and cell biology are intimately intertwined, with new visualization
possibilities often leading to dramatic leaps in our understanding of how cells function. The …

We review new and established methods for the chemical modification of proteins in living
cells and highlight recent applications. The review focuses on tag-mediated protein labeling …

Super-resolution imaging methods now can provide spatial resolution that is well below the
diffraction limit approaching virtually molecular resolution. They can be applied to biological …

A straightforward method to achieve super-resolution consists of taking an image sequence
of stochastically blinking emitters using a standard wide-field fluorescence microscope …

Assessment of protein dynamics in living cells is crucial for understanding their biological
properties and functions. The SNAP‐tag, a self labeling suicide enzyme, presents a tool with …

Technologies to visualize cellular structures and dynamics enable cell biologists to gain
insight into complex biological processes. Currently, fluorescent proteins are used routinely …

The conjugation of proteins with synthetic molecules can be conducted in many different
ways. In this Perspective, we focus on tag-based techniques and specifically on the SNAP …

Highlights•Recent advances in the design and application of live-cell imaging
probes.•Methods to visualize and quantify proteins and protein complexes in living …

Numerous synthetic fluorophores have been developed that can switch their spectroscopic
properties upon interaction with other molecules or by irradiation with light. In recent years …

Cellular compartmentalization into discrete organelles is maintained by membrane
trafficking including vesiculation and tubulation. Recent advances in superresolution …