Light sheet fluorescence microscopy (LSFM) uses a thin sheet of light to excite only
fluorophores within the focal volume. Light sheet microscopes (LSMs) have a true optical …

The impact of light-sheet fluorescence microscopy (LSFM) is visible in fields as diverse as
developmental and cell biology, anatomical science, biophysics and neuroscience …

This paper is intended to give a comprehensive review of light-sheet (LS) microscopy from
an optics perspective. As such, emphasis is placed on the advantages that LS microscope …

The tumour microenvironment (TME) shapes disease progression and influences
therapeutic response. Most aggressive solid tumours have high levels of myeloid cell …

This paper reviews the rapid advances that have been made in one form of optical biological
imaging in the last decade, namely that of light sheet microscopy. Although the concept was …

Abstract 3D cell tumour models are generated mainly in non-scalable culture systems, using
bioactive scaffolds. Many of these models fail to reflect the complex tumour …

Light-sheet fluorescence microscopy (LSFM) has been present in cell biology laboratories
for quite some time, mainly as custom-made systems, with imaging applications ranging …

There is cumulating evidence that in vitro 3D tumor models with increased physiological
relevance can improve the predictive value of pre‐clinical research and ultimately contribute …

Whole-brain imaging with light-sheet fluorescence microscopy and optically cleared tissue is
a new, rapidly developing research field. Whereas successful attempts to clear and image …

Currently there is an effort toward the development of in vitro cancer models more predictive
of clinical efficacy. The onset of advanced analytical tools and imaging technologies has …