[HTML][HTML] Cauliflower mosaic virus: Virus-host interactions and its uses in biotechnology and medicine

M Pouresmaeil, M Dall'Ara, M Salvato, V Turri, C Ratti - Virology, 2023 - Elsevier
Cauliflower mosaic virus (CaMV) was the first discovered plant virus with genomic DNA that
uses reverse transcriptase for replication. The CaMV 35S promoter is a constitutive promoter …

The dawn of plant molecular biology: how three key methodologies paved the way

M Somssich - Current Protocols, 2022 - Wiley Online Library
The adoption of Arabidopsis thaliana in the 1980s as a universal plant model finally enabled
researchers to adopt and take full advantage of the molecular biology tools and methods …

A high-efficiency Agrobacterium-mediated transient expression system in the leaves of Artemisia annua L.

Y Li, T Chen, W Wang, H Liu, X Yan, K Wu-Zhang… - Plant Methods, 2021 - Springer
Abstract Background The Agrobacterium-mediated transient transformation, which proved
effective in diverse plant species, has been widely applied for high-throughput gene function …

35S promoter-driven transgenes are variably expressed in different organs of Arabidopsis thaliana and in response to abiotic stress

KV Kiselev, OA Aleynova, ZV Ogneva… - Molecular Biology …, 2021 - Springer
The cauliflower mosaic virus (CaMV) 35S promoter is known as the most frequently used
promoter in plant biotechnology. Although it is widely considered to be a strong constitutive …

Structural feature of RrGGP2 promoter and functional analysis of RrNAC56 regulating RrGGP2 expression and ascorbate synthesis via stress-inducible cis-elements …

L Lin, S Zhang, L Luo, M Lu, H An - International Journal of Biological …, 2024 - Elsevier
Rosa roxburghii Tratt is a well-known horticultural crop that produces fruits with extremely
high l-ascorbic acid (AsA) levels, and GDP-l-galactose phosphorylase2 (RrGGP2) encodes …

CRISPRi-based circuits to control gene expression in plants

MA Khan, G Herring, JY Zhu, M Oliva, E Fourie… - Nature …, 2024 - nature.com
The construction of synthetic gene circuits in plants has been limited by a lack of orthogonal
and modular parts. Here, we implement a CRISPR (clustered regularly interspaced short …

Agrobacterium-mediated transient transformation of sorghum leaves for accelerating functional genomics and genome editing studies

R Sharma, Y Liang, MY Lee, VR Pidatala… - BMC research …, 2020 - Springer
Objectives Sorghum is one of the most recalcitrant species for transformation. Considering
the time and effort required for stable transformation in sorghum, establishing a transient …

CRISPRi-based circuits for genetic computation in plants

MA Khan, G Herring, M Oliva, E Fourie, JY Zhu… - BioRxiv, 2022 - biorxiv.org
Synthetic gene circuits can enable new cellular behaviours by integrating multiple input
signals into customisable genetic programs. However, gene circuit development in plants …

Characterization of a Stress-Enhanced Promoter from the Grass Halophyte, Spartina alterniflora L.

S Sengupta, N Pehlivan, V Mangu, K Rajasekaran… - Biology, 2022 - mdpi.com
Simple Summary Promoter is an important component to drive the expression of desirable
genes in the genetic modification of plants. Constitutive promoters that direct the expression …

An endogenous promoter LpSUT2 discovered in duckweed: a promising transgenic tool for plants

C Wei, Z Hu, S Wang, X Tan, Y Jin, Z Yi, K He… - Frontiers in Plant …, 2024 - frontiersin.org
Promoters are one of the most critical elements in regulating gene expression. They are
considered essential biotechnological tools for heterologous protein production. The one …