[HTML][HTML] Nanopore sequencing technology, bioinformatics and applications

Y Wang, Y Zhao, A Bollas, Y Wang, KF Au - Nature biotechnology, 2021 - nature.com
Rapid advances in nanopore technologies for sequencing single long DNA and RNA
molecules have led to substantial improvements in accuracy, read length and throughput …

Twenty years of plant genome sequencing: achievements and challenges

Y Sun, L Shang, QH Zhu, L Fan, L Guo - Trends in Plant Science, 2022 - cell.com
Publication of the complete genome sequence of Arabidopsis thaliana, the first plant
reference genome, in December 2000 heralded the beginning of the plant genome era …

The barley pan-genome reveals the hidden legacy of mutation breeding

M Jayakodi, S Padmarasu, G Haberer, VS Bonthala… - Nature, 2020 - nature.com
Genetic diversity is key to crop improvement. Owing to pervasive genomic structural
variation, a single reference genome assembly cannot capture the full complement of …

Efficient assembly of nanopore reads via highly accurate and intact error correction

Y Chen, F Nie, SQ Xie, YF Zheng, Q Dai, T Bray… - Nature …, 2021 - nature.com
Long nanopore reads are advantageous in de novo genome assembly. However, nanopore
reads usually have broad error distribution and high-error-rate subsequences. Existing error …

Long-read sequence assembly: a technical evaluation in barley

M Mascher, T Wicker, J Jenkins, C Plott, T Lux… - The Plant …, 2021 - academic.oup.com
Sequence assembly of large and repeat-rich plant genomes has been challenging,
requiring substantial computational resources and often several complementary sequence …

Dynamic genome evolution in a model fern

DB Marchant, G Chen, S Cai, F Chen, P Schafran… - Nature Plants, 2022 - nature.com
The large size and complexity of most fern genomes have hampered efforts to elucidate
fundamental aspects of fern biology and land plant evolution through genome-enabled …

Fast and accurate long-read assembly with wtdbg2

J Ruan, H Li - Nature methods, 2020 - nature.com
Existing long-read assemblers require thousands of central processing unit hours to
assemble a human genome and are being outpaced by sequencing technologies in terms of …

Two gap-free reference genomes and a global view of the centromere architecture in rice

JM Song, WZ Xie, S Wang, YX Guo, DH Koo, D Kudrna… - Molecular plant, 2021 - cell.com
Rice (Oryza sativa), a major staple throughout the world and a model system for plant
genomics and breeding, was the first crop genome sequenced almost two decades ago …

The genome sequence of segmental allotetraploid peanut Arachis hypogaea

DJ Bertioli, J Jenkins, J Clevenger, O Dudchenko… - Nature …, 2019 - nature.com
Like many other crops, the cultivated peanut (Arachis hypogaea L.) is of hybrid origin and
has a polyploid genome that contains essentially complete sets of chromosomes from two …

Genomic mechanisms of climate adaptation in polyploid bioenergy switchgrass

JT Lovell, AH MacQueen, S Mamidi, J Bonnette… - Nature, 2021 - nature.com
Long-term climate change and periodic environmental extremes threaten food and fuel
security and global crop productivity,–. Although molecular and adaptive breeding strategies …