Connecting past and present: single-cell lineage tracing
C Chen, Y Liao, G Peng - Protein & Cell, 2022 - academic.oup.com
Central to the core principle of cell theory, depicting cells' history, state and fate is a
fundamental goal in modern biology. By leveraging clonal analysis and single-cell RNA-seq …
fundamental goal in modern biology. By leveraging clonal analysis and single-cell RNA-seq …
Impact of CRISPR/HDR editing versus lentiviral transduction on long-term engraftment and clonal dynamics of HSPCs in rhesus macaques
For precise genome editing via CRISPR/homology-directed repair (HDR), effective and safe
editing of long-term engrafting hematopoietic stem cells (LT-HSCs) is required. The impact …
editing of long-term engrafting hematopoietic stem cells (LT-HSCs) is required. The impact …
Extracting, filtering and simulating cellular barcodes using CellBarcode tools
Identifying true DNA cellular barcodes among polymerase chain reaction and sequencing
errors is challenging. Current tools are restricted in the diversity of barcode types supported …
errors is challenging. Current tools are restricted in the diversity of barcode types supported …
Expanded NK cells used for adoptive cell therapy maintain diverse clonality and contain long-lived memory-like NK cell populations
DSJ Allan, C Wu, RD Mortlock, M Chakraborty… - Molecular Therapy …, 2023 - cell.com
Multiple clinical trials exploring the potential of adoptive natural killer (NK) cell therapy for
cancer have employed ex vivo expansion using feeder cells to obtain large numbers of NK …
cancer have employed ex vivo expansion using feeder cells to obtain large numbers of NK …
ISAnalytics enables longitudinal and high-throughput clonal tracking studies in hematopoietic stem cell gene therapy applications
G Pais, G Spinozzi, D Cesana… - Briefings in …, 2023 - academic.oup.com
Longitudinal clonal tracking studies based on high-throughput sequencing technologies
supported safety and long-term efficacy and unraveled hematopoietic reconstitution in many …
supported safety and long-term efficacy and unraveled hematopoietic reconstitution in many …
Analysis of synthetic cellular barcodes in the genome and transcriptome with BARtab and bartools
H Holze, L Talarmain, KA Fennell, EY Lam… - Cell Reports …, 2024 - cell.com
Cellular barcoding is a lineage-tracing methodology that couples heritable synthetic
barcodes to high-throughput sequencing, enabling the accurate tracing of cell lineages …
barcodes to high-throughput sequencing, enabling the accurate tracing of cell lineages …
CellDestiny: A RShiny application for the visualization and analysis of single-cell lineage tracing data
Single-cell lineage tracing permits the labeling of individual cells with a heritable marker to
follow the fate of each cell's progeny. Over the last twenty years, several single-cell lineage …
follow the fate of each cell's progeny. Over the last twenty years, several single-cell lineage …
Comparison of busulfan and total body irradiation conditioning on hematopoietic clonal dynamics following lentiviral gene transfer in rhesus macaques
DM Abraham, RJ Lozano, X Guitart, JA Liang… - … Therapy Methods & …, 2023 - cell.com
The clonal dynamics following hematopoietic stem progenitor cell (HSPC) transplantation
with busulfan conditioning are of great interest to the development of HSPC gene therapies …
with busulfan conditioning are of great interest to the development of HSPC gene therapies …
Barcode clonal tracking of tissue-resident immune cells in rhesus macaque highlights distinct clonal distribution pattern of tissue NK cells
Tissue resident (TR) immune cells play important roles in facilitating tissue homeostasis,
coordinating immune responses against infections and tumors, and maintaining …
coordinating immune responses against infections and tumors, and maintaining …
A temporal developmental map separates human NK cells from noncytotoxic ILCs through clonal and single-cell analysis
Natural killer (NK) cells represent the cytotoxic member within the innate lymphoid cell (ILC)
family that are important against viral infections and cancer. Although the NK cell emergence …
family that are important against viral infections and cancer. Although the NK cell emergence …