Chromatin is organized into higher-order structures that form subcompartments in
interphase nuclei. Different categories of specialized enzymes act on chromatin and …

Mating type in Saccharomyces cerevisiae is determined by two nonhomologous alleles,
MAT a and MAT α. These sequences encode regulators of the two different haploid mating …

DNA double-strand breaks (DSBs) are among the most deleterious forms of DNA lesions in
cells. Here we induced site-specific DSBs in yeast cells and monitored chromatin dynamics …

Chromosome dynamics are recognized to be intimately linked to genomic transactions, yet
the physical principles governing spatial fluctuations of chromatin are still a matter of debate …

Budding yeast, like other eukaryotes, carries its genetic information on chromosomes that
are sequestered from other cellular constituents by a double membrane, which forms the …

Mechanistic analyses based on improved imaging techniques have begun to explore the
biological implications of chromatin movement within the nucleus. Studies in both …

Several imaging techniques are used in biological and biomedical studies. Micro-computed
tomography (micro-CT) is a non-destructive imaging technique that allows the rapid …

High-resolution imaging shows that persistent DNA damage in budding yeast localizes in
distinct perinuclear foci for repair. The signals that trigger DNA double-strand break (DSB) …

Chromosome breakage is a major threat to genome integrity. The most accurate way to
repair DNA double strand breaks (DSB) is homologous recombination (HR) with an intact …

Double-strand break repair by recombination requires a homology search. In yeast, induced
breaks move significantly more than undamaged loci. To examine whether DNA damage …