The promise and the challenges of cryo‐electron tomography
M Turk, W Baumeister - FEBS letters, 2020 - Wiley Online Library
Structural biologists have traditionally approached cellular complexity in a reductionist
manner in which the cellular molecular components are fractionated and purified before …
manner in which the cellular molecular components are fractionated and purified before …
Cryo-EM: a unique tool for the visualization of macromolecular complexity
E Nogales, SHW Scheres - Molecular cell, 2015 - cell.com
3D cryo-electron microscopy (cryo-EM) is an expanding structural biology technique that has
recently undergone a quantum leap progression in its achievable resolution and its …
recently undergone a quantum leap progression in its achievable resolution and its …
Isotropic reconstruction for electron tomography with deep learning
Cryogenic electron tomography (cryoET) allows visualization of cellular structures in situ.
However, anisotropic resolution arising from the intrinsic “missing-wedge” problem has …
However, anisotropic resolution arising from the intrinsic “missing-wedge” problem has …
TomoTwin: generalized 3D localization of macromolecules in cryo-electron tomograms with structural data mining
G Rice, T Wagner, M Stabrin, O Sitsel, D Prumbaum… - Nature …, 2023 - nature.com
Cryogenic-electron tomography enables the visualization of cellular environments in
extreme detail, however, tools to analyze the full amount of information contained within …
extreme detail, however, tools to analyze the full amount of information contained within …
Intratomo: self-supervised learning-based tomography via sinogram synthesis and prediction
We propose IntraTomo, a powerful framework that combines the benefits of learning-based
and model-based approaches for solving highly ill-posed inverse problems in the Computed …
and model-based approaches for solving highly ill-posed inverse problems in the Computed …
[HTML][HTML] An introduction to sample preparation and imaging by cryo-electron microscopy for structural biology
Transmission electron microscopy (EM) is a versatile technique that can be used to image
biological specimens ranging from intact eukaryotic cells to individual proteins> 150 kDa …
biological specimens ranging from intact eukaryotic cells to individual proteins> 150 kDa …
Atomic electron tomography: 3D structures without crystals
BACKGROUND To understand material properties and functionality at the most fundamental
level, one must know the three-dimensional (3D) positions of atoms with high precision. For …
level, one must know the three-dimensional (3D) positions of atoms with high precision. For …
A technical introduction to transmission electron microscopy for soft‐matter: Imaging, possibilities, choices, and technical developments
With a significant role in material sciences, physics,(soft matter) chemistry, and biology, the
transmission electron microscope is one of the most widely applied structural analysis tool to …
transmission electron microscope is one of the most widely applied structural analysis tool to …
X-ray solution scattering (SAXS) combined with crystallography and computation: defining accurate macromolecular structures, conformations and assemblies in …
CD Putnam, M Hammel, GL Hura… - Quarterly reviews of …, 2007 - cambridge.org
Crystallography supplies unparalleled detail on structural information critical for mechanistic
analyses; however, it is restricted to describing low energy conformations of …
analyses; however, it is restricted to describing low energy conformations of …
Cryo-electron tomography: the challenge of doing structural biology in situ
Electron microscopy played a key role in establishing cell biology as a discipline, by
producing fundamental insights into cellular organization and ultrastructure. Many seminal …
producing fundamental insights into cellular organization and ultrastructure. Many seminal …