Biospecific Chemistry for Covalent Linking of Biomacromolecules

L Cao, L Wang - Chemical Reviews, 2024 - ACS Publications
Interactions among biomacromolecules, predominantly noncovalent, underpin biological
processes. However, recent advancements in biospecific chemistry have enabled the …

Evolution of Pyrrolysyl-tRNA Synthetase: From Methanogenesis to Genetic Code Expansion

NG Koch, N Budisa - Chemical Reviews, 2024 - ACS Publications
Over 20 years ago, the pyrrolysine encoding translation system was discovered in specific
archaea. Our Review provides an overview of how the once obscure pyrrolysyl-tRNA …

Genetically encoded chemical crosslinking of carbohydrate

S Li, N Wang, B Yu, W Sun, L Wang - Nature chemistry, 2023 - nature.com
Protein–carbohydrate interactions play important roles in various biological processes, such
as organism development, cancer metastasis, pathogen infection and immune response, but …

Bioorthogonal photocatalytic decaging-enabled mitochondrial proteomics

Z Huang, Z Liu, X Xie, R Zeng, Z Chen… - Journal of the …, 2021 - ACS Publications
Spatiotemporally resolved dissection of subcellular proteome is crucial to our understanding
of cellular functions in health and disease. We herein report a bioorthogonal and …

Ultrafast bioorthogonal spin-labeling and distance measurements in mammalian cells using small, genetically encoded tetrazine amino acids

S Jana, EGB Evans, HS Jang, S Zhang… - Journal of the …, 2023 - ACS Publications
Site-directed spin-labeling (SDSL)─ in combination with double electron–electron
resonance (DEER) spectroscopy─ has emerged as a powerful technique for determining …

Constructing Photoactivatable Protein with Genetically Encoded Photocaged Glutamic Acid

X Yang, L Zhao, Y Wang, Y Ji, XC Su… - Angewandte …, 2023 - Wiley Online Library
Genetically replacing an essential residue with the corresponding photocaged analogues
via genetic code expansion (GCE) constitutes a useful and unique strategy to directly and …

Genetically encoding latent bioreactive amino acids and the development of covalent protein drugs

N Wang, L Wang - Current Opinion in Chemical Biology, 2022 - Elsevier
As natural proteins generally do not bind targets in a covalent mode, the therapeutic
potential of covalent protein drugs remains largely unexplored. Recently, latent bioreactive …

Advancing protein therapeutics through proximity-induced chemistry

L Cheng, Y Wang, Y Guo, SS Zhang, H Xiao - Cell Chemical Biology, 2024 - cell.com
Recent years have seen a remarkable growth in the field of protein-based medical
treatments. Nevertheless, concerns have arisen regarding the cytotoxicity limitations, low …

Structural Determinants of Stimuli-Responsiveness in Amphiphilic Macromolecular Nano-assemblies

H Liu, HH Lu, Y Alp, R Wu, S Thayumanavan - Progress in Polymer …, 2023 - Elsevier
Stimuli-responsive nano-assemblies from amphiphilic macromolecules could undergo
controlled structural transformations and generate diverse macroscopic phenomenon under …

Genetically encoded crosslinkers to address protein–protein interactions

Y Aydin, I Coin - Protein Science, 2023 - Wiley Online Library
Noncanonical amino acids (ncAAs) for photo‐and chemical crosslinking are powerful
biochemical tools for studying and manipulating interactions between proteins both in vitro …