Interactions among biomacromolecules, predominantly noncovalent, underpin biological
processes. However, recent advancements in biospecific chemistry have enabled the …

Over 20 years ago, the pyrrolysine encoding translation system was discovered in specific
archaea. Our Review provides an overview of how the once obscure pyrrolysyl-tRNA …

Protein–carbohydrate interactions play important roles in various biological processes, such
as organism development, cancer metastasis, pathogen infection and immune response, but …

Spatiotemporally resolved dissection of subcellular proteome is crucial to our understanding
of cellular functions in health and disease. We herein report a bioorthogonal and …

Site-directed spin-labeling (SDSL)─ in combination with double electron–electron
resonance (DEER) spectroscopy─ has emerged as a powerful technique for determining …

Genetically replacing an essential residue with the corresponding photocaged analogues
via genetic code expansion (GCE) constitutes a useful and unique strategy to directly and …

As natural proteins generally do not bind targets in a covalent mode, the therapeutic
potential of covalent protein drugs remains largely unexplored. Recently, latent bioreactive …

Recent years have seen a remarkable growth in the field of protein-based medical
treatments. Nevertheless, concerns have arisen regarding the cytotoxicity limitations, low …

Stimuli-responsive nano-assemblies from amphiphilic macromolecules could undergo
controlled structural transformations and generate diverse macroscopic phenomenon under …

Noncanonical amino acids (ncAAs) for photo‐and chemical crosslinking are powerful
biochemical tools for studying and manipulating interactions between proteins both in vitro …