Massively parallel reporter assays (MPRAs) are powerful tools for quantifying the impacts of
sequence variation on gene expression. Reading out molecular phenotypes with …

Engineering synthetic regulatory circuits with precise input-output behavior—a central goal
in synthetic biology—remains encumbered by the inherent molecular complexity of cells …

The 5′ UTRs of mRNAs are critical for translation regulation, but their in vivo regulatory
features are poorly characterized. Here, we report the regulatory landscape of 5′ UTRs …

Cis-regulatory elements (CREs) play a pivotal role in orchestrating interactions with trans-
regulatory factors such as transcription factors, RNA-binding proteins, and noncoding RNAs …

Using mRNA as a therapeutic has received enormous attention in the last few years, but
instability of the molecule remains a hurdle to achieving long-lasting therapeutic levels of …

Concentrations of RNAs and proteins provide important determinants of cell fate. Robust
gene circuit design requires an understanding of how the combined actions of individual …

Messenger RNA (mRNA) vaccines and therapeutics are emerging as powerful tools against
a variety of diseases, including infectious diseases and cancer. The design of mRNA …

RNAs are widely regulated in cellular processes by their cis-regulatory elements and trans-
acting RNA binding proteins, the information of which is encoded in RNA sequences …

The COVID-19 pandemic accelerated the development of mRNA vaccines, yet identifying
the optimal mRNA sequence for human use, particularly for the SARS-CoV-2 spike protein …

Eukaryotic gene expression is finely regulated at the post-transcriptional level by the
untranslated regions of mRNA. The coding sequence (CDS) of mRNA is flanked by 5'-and 3' …