Molecular biologist's guide to proteomics

PR Graves, TAJ Haystead - Microbiology and molecular biology …, 2002 - Am Soc Microbiol
The emergence of proteomics, the large-scale analysis of proteins, has been inspired by the
realization that the final product of a gene is inherently more complex and closer to function …

Metabolomics in systems biology

W Weckwerth - Annual review of plant biology, 2003 - annualreviews.org
The primary aim of “omic” technologies is the nontargeted identification of all gene products
(transcripts, proteins, and metabolites) present in a specific biological sample. By their …

Protein labeling by iTRAQ: a new tool for quantitative mass spectrometry in proteome research

S Wiese, KA Reidegeld, HE Meyer, B Warscheid - Proteomics, 2007 - Wiley Online Library
A novel, MS‐based approach for the relative quantification of proteins, relying on the
derivatization of primary amino groups in intact proteins using isobaric tag for relative and …

[HTML][HTML] Proteomics: the first decade and beyond

SD Patterson, RH Aebersold - Nature genetics, 2003 - nature.com
Proteomics is the systematic study of the many and diverse properties of proteins in a
parallel manner with the aim of providing detailed descriptions of the structure, function and …

AT_CHLORO, a comprehensive chloroplast proteome database with subplastidial localization and curated information on envelope proteins

M Ferro, S Brugière, D Salvi… - Molecular & Cellular …, 2010 - ASBMB
Recent advances in the proteomics field have allowed a series of high throughput
experiments to be conducted on chloroplast samples, and the data are available in several …

Multiplexed and data‐independent tandem mass spectrometry for global proteome profiling

JD Chapman, DR Goodlett… - Mass spectrometry …, 2014 - Wiley Online Library
One of the most important early developments in the field of proteomics was the advent of
automated data acquisition routines that allowed high‐throughput unattended data …

Systems toxicology approaches for understanding the joint effects of environmental chemical mixtures

DJ Spurgeon, OAH Jones, JLCM Dorne… - Science of the total …, 2010 - Elsevier
Environmental mixtures of chemicals constitute a prevalent issue in ecotoxicology and the
development of new methods to reduce the uncertainties associated with their ecological …

18O Labeling: a tool for proteomics

II Stewart, T Thomson, D Figeys - Rapid Communications in …, 2001 - Wiley Online Library
An evaluation of the proteolytic labeling and quantification of proteins for diagnostic
purposes using trypsin and 18O‐enriched H2O is presented. We demonstrate that …

Capillary electrophoresis–mass spectrometry: 15 years of developments and applications

P Schmitt‐Kopplin, M Frommberger - Electrophoresis, 2003 - Wiley Online Library
Since its introduction in 1987, capillary electrophoresis‐mass spectrometry (CE‐MS) has
developed to a well accepted multidimensional analytical approach complementary and/or …

Proteome analysis by two-dimensional gel electrophoresis and mass spectrometry: strengths and limitations

S Beranova-Giorgianni - TrAC Trends in Analytical Chemistry, 2003 - Elsevier
Two-dimensional gel electrophoresis, mass spectrometry and bioinformatics are the key
components of current proteomics technology. The purpose of this review is to give an …