Functionalizing DNA origami to investigate and interact with biological systems

GA Knappe, EC Wamhoff, M Bathe - Nature Reviews Materials, 2023 - nature.com
DNA origami has emerged as a powerful method to generate DNA nanostructures with
dynamic properties and nanoscale control. These nanostructures enable complex …

Adoption of a Tetrahedral DNA Nanostructure as a Multifunctional Biomaterial for Drug Delivery

J Huang, A Chakraborty, LS Tadepalli… - ACS Pharmacology & …, 2024 - ACS Publications
DNA nanostructures have been widely researched in recent years as emerging biomedical
materials for drug delivery, biosensing, and cancer therapy, in addition to their hereditary …

Enhanced functional properties of three DNA origami nanostructures as doxorubicin carriers to breast cancer cells

A Udomprasert, C Wootthichairangsan… - ACS Applied Bio …, 2022 - ACS Publications
Previous studies have shown that chemotherapeutic efficacy could be enhanced with
targeted drug delivery. Various DNA origami nanostructures have been investigated as drug …

CRISPR/Cas14 and G-Quadruplex DNAzyme-Driven Biosensor for Paper-Based Colorimetric Detection of African Swine Fever Virus

X Zhao, Y He, S Shao, Q Ci, L Chen, X Lu, Q Liu… - ACS …, 2024 - ACS Publications
The highly contagious nature and 100% fatality rate contribute to the ongoing and
expanding impact of the African swine fever virus (ASFV), causing significant economic …

A signal on–off strategy based on the digestion of DNA cubes assisted by the CRISPR–Cas12a system for ultrasensitive HBV detection in solid-state nanopores

J Wang, L Luo, Y Li, L Chen, C Gui, J Zhu, H Li… - Analyst, 2022 - pubs.rsc.org
Solid-state nanopores have been proven as a powerful platform for label-free single-
molecule analysis. However, due to its relatively low resolution and selectivity, developing …

Aptamer-integrated scaffolds for biologically functional DNA origami structures

X Chen, B Jia, Z Lu, L Liao, H Yu… - ACS Applied Materials & …, 2021 - ACS Publications
The manufacture of DNA origami nanostructures with highly ordered functional motifs is of
great significance for biomedical applications. Here, we present a robust strategy to produce …

ssDNA is not superior to dsDNA as long HDR donors for CRISPR-mediated endogenous gene tagging in human diploid RPE1 and HCT116 cells

A Mabuchi, S Hata, M Genova, C Tei, KK Ito, M Hirota… - BMC genomics, 2023 - Springer
Background Recent advances in CRISPR technology have enabled us to perform gene
knock-in in various species and cell lines. CRISPR-mediated knock-in requires donor DNA …

A method for generating user‐defined circular single‐stranded DNA from plasmid DNA using Golden Gate intramolecular ligation

IK Strawn, PJ Steiner, MS Newton… - Biotechnology and …, 2023 - Wiley Online Library
Construction of user‐defined long circular single stranded DNA (cssDNA) and linear single
stranded DNA (lssDNA) is important for various biotechnological applications. Many current …

Bridging biological samples to functional nucleic acid biosensor applications: current enzymatic-based strategies for single-stranded DNA generation

DSS Marpaung, AOY Sinaga, D Damayanti… - Analytical …, 2024 - Springer
The escalating threat of emerging diseases, often stemming from contaminants and lethal
pathogens, has precipitated a heightened demand for sophisticated diagnostic tools. Within …

Nucleic acid detection with single-base specificity integrating isothermal amplification and light-up aptamer probes

J Baek, J Park, Y Kim - Nanoscale, 2024 - pubs.rsc.org
We report a novel platform for label-free nucleic acid detection using isothermal
amplification and light-up aptamer probes. This assay converts double-stranded amplicons …