Aptamers 101: aptamer discovery and in vitro applications in biosensors and separations

LF Yang, M Ling, N Kacherovsky, SH Pun - Chemical Science, 2023 - pubs.rsc.org
Aptamers are single-stranded nucleic acids that bind and recognize targets much like
antibodies. Recently, aptamers have garnered increased interest due to their unique …

[HTML][HTML] Three decades of nucleic acid aptamer technologies: Lessons learned, progress and opportunities on aptamer development

T Wang, C Chen, LM Larcher, RA Barrero… - Biotechnology …, 2019 - Elsevier
Aptamers are short single-stranded nucleic acid sequences capable of binding to target
molecules in a way similar to antibodies. Due to various advantages such as prolonged …

Engineering synthetic RNA devices for cell control

PB Dykstra, M Kaplan, CD Smolke - Nature Reviews Genetics, 2022 - nature.com
The versatility of RNA in sensing and interacting with small molecules, proteins and other
nucleic acids while encoding genetic instructions for protein translation makes it a powerful …

Functional DNA molecules enable selective and stimuli-responsive nanoparticles for biomedical applications

L Li, H Xing, J Zhang, Y Lu - Accounts of chemical research, 2019 - ACS Publications
Conspectus Nanoparticles (NPs) have enormous potential to improve disease diagnosis
and treatment due to their intrinsic electronic, optical, magnetic, mechanical, and …

Do aptamers always bind? The need for a multifaceted analytical approach when demonstrating binding affinity between aptamer and low molecular weight …

F Bottari, E Daems, AM de Vries… - Journal of the …, 2020 - ACS Publications
In this manuscript, we compare different analytical methodologies to validate or disprove the
binding capabilities of aptamer sequences. This was prompted by the lack of a universally …

Immobilization techniques for aptamers on gold electrodes for the electrochemical detection of proteins: a review

FV Oberhaus, D Frense, D Beckmann - Biosensors, 2020 - mdpi.com
The development of reliable biosensing platforms plays a key role in the detection of
proteins in clinically and environmentally derived samples for diagnostics, as well as for …

Nucleic acid and peptide aptamers: fundamentals and bioanalytical aspects

M Mascini, I Palchetti, S Tombelli - … Chemie International Edition, 2012 - Wiley Online Library
In recent years new nucleic acid and protein‐based combinatorial molecules have attracted
the attention of researchers working in various areas of science, ranging from medicine to …

Aptamer modules as sensors and detectors

M Famulok, G Mayer - Accounts of chemical research, 2011 - ACS Publications
Aptamers comprise a range of molecular recognition scaffolds that can be engineered to
bind to a legion of different proteins and other targets with excellent specificity and affinity …

Anything you can do, I can do better: Can aptamers replace antibodies in clinical diagnostic applications?

M Bauer, M Strom, DS Hammond, S Shigdar - Molecules, 2019 - mdpi.com
The mainstay of clinical diagnostics is the use of specialised ligands that can recognise
specific biomarkers relating to pathological changes. While protein antibodies have been …

Analysis of in vitro aptamer selection parameters

M McKeague, EM McConnell, J Cruz-Toledo… - Journal of Molecular …, 2015 - Springer
Nucleic acid aptamers are novel molecular recognition tools that offer many advantages
compared to their antibody and peptide-based counterparts. However, challenges …