[HTML][HTML] Methods for genetic transformation of filamentous fungi

D Li, Y Tang, J Lin, W Cai - Microbial cell factories, 2017 - Springer
Filamentous fungi have been of great interest because of their excellent ability as cell
factories to manufacture useful products for human beings. The development of genetic …

Premeiotic instability of repeated sequences in Neurospora crassa.

EU Selker - Annual review of genetics, 1990 - europepmc.org
Maintenance of a steamlined genome is probably important to a free-living fungus. The
period between fertilization and karyogamy in the life cycle of Neurospora and related fungi …

A high-throughput gene knockout procedure for Neurospora reveals functions for multiple transcription factors

HV Colot, G Park, GE Turner… - Proceedings of the …, 2006 - National Acad Sciences
The low rate of homologous recombination exhibited by wild-type strains of filamentous
fungi has hindered development of high-throughput gene knockout procedures for this …

A versatile transformation system for the cellulolytic filamentous fungus Trichoderma reesei

M Penttilä, H Nevalainen, M Rättö, E Salminen… - Gene, 1987 - Elsevier
An efficient transformation system for the cellulolytic filamentous fungus Trichoderma reesei
has been developed. Transformation was obtained with plasmid carrying the dominant …

Transformation of Aspergillus nidulans by using a trpC plasmid.

MM Yelton, JE Hamer… - Proceedings of the …, 1984 - National Acad Sciences
We constructed a chimeric plasmid carrying a complete copy of the trifunctional trpC gene
from the Ascomycete fungus Aspergillus nidulans. This plasmid, designated pHY201 …

Transformation in fungi

JR Fincham - Microbiological reviews, 1989 - Am Soc Microbiol
Transformation with exogenous deoxyribonucleic acid (DNA) now appears to be possible
with all fungal species, or at least all that can be grown in culture. This field of research is at …

Transformation of Aspergillus niger by the amdS gene of Aspergillus nidulans.

JM Kelly, MJ Hynes - The EMBO journal, 1985 - embopress.org
Aspergillus niger grows poorly on acetamide as a nitrogen or carbon source and lacks
sequences detectably homologous to the amdS gene encoding the acetamidase of …

[39] Transformation of filamentous fungi based on hygromycin b and phleomycin resistance markers

PJ Punt, CA van den Hondel - Methods in enzymology, 1992 - Elsevier
Publisher Summary This chapter describes fungal transformation based on the HmB
resistance gene of E. coli (hph) and the phleomycin resistance gene of S. hindustanus (ble) …

Repeat‐induced point mutation and other genome defense mechanisms in fungi

E Gladyshev - The fungal kingdom, 2017 - Wiley Online Library
Mobile DNA, comprising both active and decaying copies of transposable elements (TEs), is
present in nearly all living organisms. Although fungal genomes tend to be significantly …

Transformation of Aspergillus nidulans by the orotidine-5′-phosphate decarboxylase gene of Neurospora crassa

DJ Ballance, FP Buxton, G Turner - Biochemical and biophysical research …, 1983 - Elsevier
Relief of an auxotrophic requirement for uridine in Aspergillus nidulans strain G191 has
been achieved by transformation with a segment of Neurospora crassa DNA containing the …