Escherichia coli as a versatile cell factory: Advances and challenges in recombinant protein production

İ İncir, Ö Kaplan - Protein Expression and Purification, 2024 - Elsevier
E. coli plays a substantial role in recombinant protein production. Its importance increased
with the discovery of recombinant DNA technology and the subsequent production of the first …

Challenges in recombinant brain-derived neurotrophic factor production

ÉF Rodrigues, AL Fachin, M Marins, RG Stabeli… - Trends in …, 2023 - cell.com
Brain-derived neurotrophic factor (BDNF) is a neurotrophin of marked commercial, scientific,
diagnostic, and therapeutic interest. The preservation of its structural cystine-knot is the main …

Enzymatic Modification of Starch Using Recombinant Genes from Sorghum in Escherichia coli: Insights and Potential Applications

X Kang, W Gao, Y Cheng, B Cui, X Qiao… - Journal of Agricultural …, 2023 - ACS Publications
SBEIIb (Sobic. 004G163700), SSSIIa (Sobic. 010G093400), and GBSSI (Sobic.
010G022600) genes that regulate starch synthesis in sorghum endosperm were transferred …

Rational design of soluble expressed human aldehyde dehydrogenase 2 with high stability and activity in pepsin and trypsin

M Hu, JX Song, ST Miao, CK Wu, XW Gong… - International Journal of …, 2024 - Elsevier
Abstract Acetaldehyde dehydrogenase 2 (ALDH2) is a crucial enzyme in alcohol
metabolism, and oral administration of ALDH2 is a promising method for alcohol …

Human super antibody to viral RNA-dependent RNA polymerase produced by a modified Sortase self-cleave-bacteria surface display system

K Glab-Ampai, K Mahasongkram, M Chulanetra… - Microbial Cell …, 2023 - Springer
Background RNA-dependent RNA polymerase (RdRp) is a good target of anti-RNA virus
agents; not only it is pivotal for the RNA virus replication cycle and highly conserved among …

[HTML][HTML] Engineering and finetuning expression of SerC for balanced metabolic flux in vitamin B6 production

K Chen, L Liu, J Li, Z Tian, H Jin, D Zhang - Synthetic and Systems …, 2024 - Elsevier
Vitamin B 6 plays a crucial role in cellular metabolism and stress response, making it an
essential component for growth in all known organisms. However, achieving efficient …

Evolving Paradigms of Recombinant Protein Production in Pharmaceutical Industry: A Rigorous Review

A Jayakrishnan, WR Wan Rosli, ARM Tahir, FSA Razak… - Sci, 2024 - mdpi.com
Many beneficial proteins have limited natural availability, which often restricts their supply
and thereby reduces their potential for therapeutic or industrial usage. The advent of …

Engineering and Expression Strategies for Optimization of L-Asparaginase Development and Production

AN Shishparenok, YA Gladilina… - International Journal of …, 2023 - mdpi.com
Genetic engineering for heterologous expression has advanced in recent years. Model
systems such as Escherichia coli, Bacillus subtilis and Pichia pastoris are often used as host …

Komagataella phaffii as a Platform for Heterologous Expression of Enzymes Used for Industry

TM Khlebodarova, NV Bogacheva, AV Zadorozhny… - Microorganisms, 2024 - mdpi.com
In the 1980s, Escherichia coli was the preferred host for heterologous protein expression
owing to its capacity for rapid growth in complex media; well-studied genetics; rapid and …

Cloning, Expression, Purification, and Characterization of Lactate Dehydrogenase from Plasmodium knowlesi: A Zoonotic Malaria Parasite

JW Choi, MJ Choi, YJ Kim, SY Kim - International Journal of Molecular …, 2024 - mdpi.com
Plasmodium knowlesi is the only Plasmodium that causes zoonotic disease among the
Plasmodium that cause infection in humans. It is fatal due to its short asexual growth cycle …