When a molecule absorbs a photon of appropriate energy, a chain of photophysical events
ensues, such as internal conversion or vibrational relaxation (loss of energy in the absence …

Fluorescence lifetime imaging (FLIM) is a key fluorescence microscopy technique to map the
environment and interaction of fluorescent probes. It can report on photophysical events that …

The Kohlrausch (stretched exponential) decay law is analyzed in detail. Analytical and
approximate forms of the distribution of rate constants and related functions are obtained for …

In fluorescence microscopy, the fluorescence emission can be characterised not only by
intensity and position, but also by lifetime, polarization and wavelength. Fluorescence …

Fluorescence lifetime imaging microscopy (FLIM) is a technique to map the spatial
distribution of nanosecond excited state lifetimes within microscopic images. FLIM systems …

Objective Fluorescence guidance has a demonstrated potential in maximizing the extent of
high-grade glioma resection. Different fluorophores (fluorescent biomarkers), including 5 …

Cancer metastasis involves complex cell behavior and interaction with the extracellular
matrix by metabolically active cells. To observe invasion and metastasis with sub-cellular …

The study of biological systems in their real environmental conditions is crucial to decipher
the true image of structures and processes underlying their functionality. In this regard …

Fluorescence lifetime imaging (FLIM) is a functional imaging methodology that can provide
information, not only concerning the localisation of specific fluorophores, but also about the …

Publisher Summary Fluorescence lifetime imaging microscopy (FLIM) produces spatially
resolved images of fluorophore lifetime (the property describing how rapidly fluorescence …