Methodological trends and perspectives of animal dietary studies by noninvasive fecal DNA metabarcoding

H Ando, H Mukai, T Komura, T Dewi… - Environmental …, 2020 - Wiley Online Library
Fine‐scale estimation of trophic interactions is an important subject in the field of ecology.
Diet analysis based on fecal DNA metabarcoding has been accepted as a noninvasive …

Promises and pitfalls of using high‐throughput sequencing for diet analysis

A Alberdi, O Aizpurua, K Bohmann… - Molecular ecology …, 2019 - Wiley Online Library
The application of high‐throughput sequencing‐based approaches to DNA extracted from
environmental samples such as gut contents and faeces has become a popular tool for …

An assessment of minimum sequence copy thresholds for identifying and reducing the prevalence of artefacts in dietary metabarcoding data

LE Drake, JP Cuff, RE Young… - Methods in Ecology …, 2022 - Wiley Online Library
Metabarcoding provides a powerful tool for investigating biodiversity and trophic
interactions, but the high sensitivity of this methodology makes it vulnerable to errors …

DNA metabarcoding multiplexing and validation of data accuracy for diet assessment: application to omnivorous diet

M De Barba, C Miquel, F Boyer… - Molecular ecology …, 2014 - Wiley Online Library
Ecological understanding of the role of consumer–resource interactions in natural food webs
is limited by the difficulty of accurately and efficiently determining the complex variety of food …

Metabarcoding for the parallel identification of several hundred predators and their prey: Application to bat species diet analysis

M Galan, JB Pons, O Tournayre, E Pierre… - Molecular ecology …, 2018 - Wiley Online Library
Assessing diet variability is of main importance to better understand the biology of bats and
design conservation strategies. Although the advent of metabarcoding has facilitated such …

Current methods and future directions in avian diet analysis

BD Hoenig, AM Snider, AM Forsman, KA Hobson… - The Auk, 2022 - academic.oup.com
Identifying the composition of avian diets is a critical step in characterizing the roles of birds
within ecosystems. However, because birds are a diverse taxonomic group with equally …

Persistence of DNA in carcasses, slime and avian feces may affect interpretation of environmental DNA data

CM Merkes, SG McCalla, NR Jensen, MP Gaikowski… - PLoS …, 2014 - journals.plos.org
The prevention of non-indigenous aquatic invasive species spreading into new areas is a
goal of many resource managers. New techniques have been developed to survey for …

Advancing the integration of multi‐marker metabarcoding data in dietary analysis of trophic generalists

LP da Silva, VA Mata, PB Lopes… - Molecular Ecology …, 2019 - Wiley Online Library
The application of DNA metabarcoding to dietary analysis of trophic generalists requires
using multiple markers in order to overcome problems of primer specificity and bias …

The future of environmental DNA in ecology

NG Yoccoz - Molecular ecology, 2012 - Wiley Online Library
The contributions of environmental DNA to ecology are reviewed, focusing on diet, trophic
interactions, species distributions and biodiversity assessment. Environmental DNA has the …

Empirically characterising trophic networks: what emerging DNA-based methods, stable isotope and fatty acid analyses can offer

M Traugott, S Kamenova, L Ruess, J Seeber… - Advances in ecological …, 2013 - Elsevier
Food webs in agricultural systems are complex and trophic linkages are difficult to track
using conventional methodologies. Here, we review three alternative approaches that allow …