Sequencing technologies and genome sequencing
The high-throughput-next generation sequencing (HT-NGS) technologies are currently the
hottest topic in the field of human and animals genomics researches, which can produce …
hottest topic in the field of human and animals genomics researches, which can produce …
Digital microfluidics: is a true lab-on-a-chip possible?
RB Fair - Microfluidics and Nanofluidics, 2007 - Springer
The suitability of electrowetting-on-dielectric (EWD) microfluidics for true lab-on-a-chip
applications is discussed. The wide diversity in biomedical applications can be parsed into …
applications is discussed. The wide diversity in biomedical applications can be parsed into …
Highly multiplexed subcellular RNA sequencing in situ
Understanding the spatial organization of gene expression with single-nucleotide resolution
requires localizing the sequences of expressed RNA transcripts within a cell in situ. Here …
requires localizing the sequences of expressed RNA transcripts within a cell in situ. Here …
Detection and quantification of rare mutations with massively parallel sequencing
I Kinde, J Wu, N Papadopoulos… - Proceedings of the …, 2011 - National Acad Sciences
The identification of mutations that are present in a small fraction of DNA templates is
essential for progress in several areas of biomedical research. Although massively parallel …
essential for progress in several areas of biomedical research. Although massively parallel …
Safe sequencing system
B Vogelstein, KW Kinzler, N Papadopoulos… - US Patent …, 2016 - Google Patents
The identification of mutations that are present in a small fraction of DNA templates is
essential for progress in several areas of biomedical research. Though massively parallel …
essential for progress in several areas of biomedical research. Though massively parallel …
Transforming single DNA molecules into fluorescent magnetic particles for detection and enumeration of genetic variations
Many areas of biomedical research depend on the analysis of uncommon variations in
individual genes or transcripts. Here we describe a method that can quantify such variation …
individual genes or transcripts. Here we describe a method that can quantify such variation …
Whole-genome molecular haplotyping of single cells
Conventional experimental methods of studying the human genome are limited by the
inability to independently study the combination of alleles, or haplotype, on each of the …
inability to independently study the combination of alleles, or haplotype, on each of the …
Advanced sequencing technologies: methods and goals
J Shendure, RD Mitra, C Varma, GM Church - Nature Reviews Genetics, 2004 - nature.com
Nearly three decades have passed since the invention of electrophoretic methods for DNA
sequencing. The exponential growth in the cost-effectiveness of sequencing has been …
sequencing. The exponential growth in the cost-effectiveness of sequencing has been …
Haplotype-resolved genome sequencing: experimental methods and applications
MW Snyder, A Adey, JO Kitzman… - Nature Reviews Genetics, 2015 - nature.com
Human genomes are diploid and, for their complete description and interpretation, it is
necessary not only to discover the variation they contain but also to arrange it onto …
necessary not only to discover the variation they contain but also to arrange it onto …
Fluid control structures in microfluidic devices
RA Mathies, WH Grover, A Skelley, E Lagally… - US Patent …, 2008 - Google Patents
Methods and apparatus for implementing microfluidic analysis devices are provided. A
monolithic elastomer membrane associated with an integrated pneumatic manifold allows …
monolithic elastomer membrane associated with an integrated pneumatic manifold allows …