Dynamics of E. coli single stranded DNA binding (SSB) protein-DNA complexes

E Antony, TM Lohman - Seminars in cell & developmental biology, 2019 - Elsevier
Single stranded DNA binding proteins (SSB) are essential to the cell as they stabilize
transiently open single stranded DNA (ssDNA) intermediates, recruit appropriate DNA …

The mechanism of action of the SSB interactome reveals it is the first OB‐fold family of genome guardians in prokaryotes

PR Bianco - Protein science, 2021 - Wiley Online Library
The single‐stranded DNA binding protein (SSB) is essential to all aspects of DNA
metabolism in bacteria. This protein performs two distinct, but closely intertwined and …

Phase separation by ssDNA binding protein controlled via protein− protein and protein− DNA interactions

GM Harami, ZJ Kovács, R Pancsa… - Proceedings of the …, 2020 - National Acad Sciences
Bacterial single-stranded (ss) DNA-binding proteins (SSB) are essential for the replication
and maintenance of the genome. SSBs share a conserved ssDNA-binding domain, a less …

How glutamate promotes liquid-liquid phase separation and DNA binding cooperativity of E. coli SSB protein

AG Kozlov, X Cheng, H Zhang, MK Shinn… - Journal of molecular …, 2022 - Elsevier
E. coli single-stranded-DNA binding protein (EcSSB) displays nearest-neighbor (NN) and
non-nearest-neighbor (NNN)) cooperativity in binding ssDNA during genome maintenance …

Defining single molecular forces required for notch activation using nano yoyo

F Chowdhury, ITS Li, TTM Ngo, BJ Leslie, BC Kim… - Nano …, 2016 - ACS Publications
Notch signaling, involved in development and tissue homeostasis, is activated at the cell–
cell interface through ligand–receptor interactions. Previous studies have implicated …

The Intrinsically Disordered C-terminal Tails of E. coli Single-Stranded DNA Binding Protein Regulate Cooperative Binding to Single-Stranded DNA

AG Kozlov, E Weiland, A Mittal, V Waldman… - Biophysical …, 2015 - cell.com
E. coli single strand DNA binding protein (SSB) is one of the key proteins in DNA replication,
recombination and repair. SSB functions as a homotetramer and binds ssDNA in different …

Replication stalling activates SSB for recruitment of DNA damage tolerance factors

ES Thrall, SC Piatt, S Chang… - Proceedings of the …, 2022 - National Acad Sciences
Translesion synthesis (TLS) polymerases bypass DNA lesions that block replicative
polymerases, allowing cells to tolerate DNA damage encountered during replication. It is …

Multiprotein E. coli SSB–ssDNA complex shows both stable binding and rapid dissociation due to interprotein interactions

MN Naufer, M Morse, GB Möller, J McIsaac… - Nucleic acids …, 2021 - academic.oup.com
Abstract Escherichia coli SSB (Ec SSB) is a model single-stranded DNA (ssDNA) binding
protein critical in genome maintenance. Ec SSB forms homotetramers that wrap ssDNA in …

Role of protein phosphorylation in the regulation of cell cycle and DNA-related processes in bacteria

T Garcia-Garcia, S Poncet, A Derouiche, L Shi… - Frontiers in …, 2016 - frontiersin.org
In all living organisms, the phosphorylation of proteins modulates various aspects of their
functionalities. In eukaryotes, protein phosphorylation plays a key role in cell signaling, gene …

The E. coli DNA replication fork

JS Lewis, S Jergic, NE Dixon - The Enzymes, 2016 - Elsevier
DNA replication in Escherichia coli initiates at oriC, the origin of replication and proceeds
bidirectionally, resulting in two replication forks that travel in opposite directions from the …