Isothermal amplification of nucleic acids
Isothermal amplification of nucleic acids is a simple process that rapidly and efficiently
accumulates nucleic acid sequences at constant temperature. Since the early 1990s …
accumulates nucleic acid sequences at constant temperature. Since the early 1990s …
Intratumor heterogeneity in breast cancer
F Beca, K Polyak - Novel biomarkers in the continuum of breast cancer, 2016 - Springer
Intratumor heterogeneity is the main obstacle to effective cancer treatment and personalized
medicine. Both genetic and epigenetic sources of intratumor heterogeneity are well …
medicine. Both genetic and epigenetic sources of intratumor heterogeneity are well …
Spatial imaging of glycoRNA in single cells with ARPLA
Little is known about the biological roles of glycosylated RNAs (glycoRNAs), a recently
discovered class of glycosylated molecules, because of a lack of visualization methods. We …
discovered class of glycosylated molecules, because of a lack of visualization methods. We …
A combined approach for single-cell mRNA and intracellular protein expression analysis
Combined measurements of mRNA and protein expression in single cells enable in-depth
analysis of cellular states. We present SPARC, an approach that combines single-cell RNA …
analysis of cellular states. We present SPARC, an approach that combines single-cell RNA …
Highly multiplexed simultaneous detection of RNAs and proteins in single cells
To enable the detection of expression signatures specific to individual cells, we developed
PLAYR (proximity ligation assay for RNA), a method for highly multiplexed transcript …
PLAYR (proximity ligation assay for RNA), a method for highly multiplexed transcript …
Single cell–resolution western blotting
This protocol describes how to perform western blotting on individual cells to measure cell-to-
cell variation in protein expression levels and protein state. Like conventional western …
cell variation in protein expression levels and protein state. Like conventional western …
Direct visualization of single-nucleotide variation in mtDNA using a CRISPR/Cas9-mediated proximity ligation assay
The accumulation of mitochondrial DNA (mtDNA) mutations in cells is strongly related to
aging-associated diseases. Imaging of single-nucleotide variation (SNV) in mtDNA is crucial …
aging-associated diseases. Imaging of single-nucleotide variation (SNV) in mtDNA is crucial …
Rolling circle amplification in integrated microsystems: An uncut gem toward massively multiplexed pathogen diagnostics and genotyping
RRG Soares, N Madaboosi… - Accounts of Chemical …, 2021 - ACS Publications
Conspectus The development of robust methods allowing the precise detection of specific
nucleic acid sequences is of major societal relevance, paving the way for significant …
nucleic acid sequences is of major societal relevance, paving the way for significant …
Automated cell-type classification in intact tissues by single-cell molecular profiling
M Nagendran, DP Riordan, PB Harbury, TJ Desai - Elife, 2018 - elifesciences.org
A major challenge in biology is identifying distinct cell classes and mapping their
interactions in vivo. Tissue-dissociative technologies enable deep single cell molecular …
interactions in vivo. Tissue-dissociative technologies enable deep single cell molecular …
Rolling circle amplification for single cell analysis and in situ sequencing
Single cell analysis is of great significance to biological and biomedical research. With
recent development, lots of in situ amplification methods have been applied for imaging the …
recent development, lots of in situ amplification methods have been applied for imaging the …