The alpha-gal story: lessons learned from connecting the dots
JW Steinke, TAE Platts-Mills, SP Commins - Journal of Allergy and Clinical …, 2015 - Elsevier
Anaphylaxis is a severe allergic reaction that can be rapidly progressing and fatal, and
therefore establishing its cause is pivotal to long-term risk management. Our recent work has …
therefore establishing its cause is pivotal to long-term risk management. Our recent work has …
Chromatographic, electrophoretic, and mass spectrometric methods for the analytical characterization of protein biopharmaceuticals
Protein biopharmaceuticals are macromolecules with a therapeutic effect, commonly
produced by the recombinant DNA technology. They have emerged as important …
produced by the recombinant DNA technology. They have emerged as important …
Specific targeting of glioblastoma with an oncolytic virus expressing a cetuximab-CCL5 fusion protein via innate and adaptive immunity
Chemokines such as CC motif ligand 5 (CCL5) regulate immune cell trafficking in the tumor
microenvironment (TME) and govern tumor development, making them promising targets for …
microenvironment (TME) and govern tumor development, making them promising targets for …
Amivantamab (JNJ-61186372), an Fc enhanced EGFR/cMet bispecific antibody, induces receptor downmodulation and antitumor activity by monocyte/macrophage …
S Vijayaraghavan, L Lipfert, K Chevalier… - Molecular cancer …, 2020 - AACR
Small molecule inhibitors targeting mutant EGFR are standard of care in non–small cell lung
cancer (NSCLC), but acquired resistance invariably develops through mutations in EGFR or …
cancer (NSCLC), but acquired resistance invariably develops through mutations in EGFR or …
Ion-exchange chromatography for the characterization of biopharmaceuticals
Ion-exchange chromatography (IEX) is a historical technique widely used for the detailed
characterization of therapeutic proteins and can be considered as a reference and powerful …
characterization of therapeutic proteins and can be considered as a reference and powerful …
Immunoglobulin G (IgG) Fab glycosylation analysis using a new mass spectrometric high-throughput profiling method reveals pregnancy-associated changes
A Bondt, Y Rombouts, MHJ Selman… - Molecular & Cellular …, 2014 - ASBMB
The N-linked glycosylation of the constant fragment (Fc) of immunoglobulin G has been
shown to change during pathological and physiological events and to strongly influence …
shown to change during pathological and physiological events and to strongly influence …
Rapid Preparation of Released N-Glycans for HILIC Analysis Using a Labeling Reagent that Facilitates Sensitive Fluorescence and ESI-MS Detection
MA Lauber, YQ Yu, DW Brousmiche, Z Hua… - Analytical …, 2015 - ACS Publications
N-glycosylation of proteins is now routinely characterized and monitored because of its
significance to the detection of disease states and the manufacturing of biopharmaceuticals …
significance to the detection of disease states and the manufacturing of biopharmaceuticals …
The impact II, a very high-resolution quadrupole time-of-flight instrument (QTOF) for deep shotgun proteomics
S Beck, A Michalski, O Raether, M Lubeck… - Molecular & Cellular …, 2015 - ASBMB
Hybrid quadrupole time-of-flight (QTOF) mass spectrometry is one of the two major
principles used in proteomics. Although based on simple fundamentals, it has over the last …
principles used in proteomics. Although based on simple fundamentals, it has over the last …
Multi-dimensional LC-MS: the next generation characterization of antibody-based therapeutics by unified online bottom-up, middle-up and intact approaches
Accelerated development of new therapeutics in an increasingly competitive landscape
requires the use of high throughput analytical platforms. In addition, the complexity of novel …
requires the use of high throughput analytical platforms. In addition, the complexity of novel …