Exosomal miR-10a derived from amniotic fluid stem cells preserves ovarian follicles after chemotherapy

GY Xiao, CC Cheng, YS Chiang, WTK Cheng, IH Liu… - Scientific reports, 2016 - nature.com
Chemotherapy (CTx)-induced premature ovarian failure (POF) in woman remains clinically
irreversible. Amniotic fluid stem cells (AFSCs) have shown the potential to treat CTx-induced …

Amniotic fluid stem cells prevent follicle atresia and rescue fertility of mice with premature ovarian failure induced by chemotherapy

GY Xiao, IH Liu, CC Cheng, CC Chang, YH Lee… - PLoS …, 2014 - journals.plos.org
Chemotherapy used to treat cancer may cause irreversible premature ovarian failure (POF).
Of late, amniotic fluid stem cells (AFSCs) provide a novel source for regenerative medicine …

[HTML][HTML] Next generation tools for high-throughput promoter and expression analysis employing single-copy knock-ins at the Hprt1 locus

GS Yang, KG Banks, RJ Bonaguro, G Wilson… - Genomics, 2009 - Elsevier
We have engineered a set of useful tools that facilitate targeted single copy knock-in (KI) at
the hypoxanthine guanine phosphoribosyl transferase 1 (Hprt1) locus. We employed fine …

Isolation of therapeutically functional mouse bone marrow mesenchymal stem cells within 3 h by an effective single‐step plastic‐adherent method

FSH Hsiao, CC Cheng, SY Peng, HY Huang… - Cell …, 2010 - Wiley Online Library
Objectives: Isolation of mouse mesenchymal stem cells (mMSCs), by the approach of plastic
adherence, has been difficult due to persistent contamination by haematopoietic cells (HCs); …

The signals of FGFs on the neurogenesis of embryonic stem cells

CW Chen, CS Liu, IM Chiu, SC Shen, HC Pan… - Journal of Biomedical …, 2010 - Springer
Background Neural induction is a complex process and the detailed mechanism of FGF-
induced neurogenesis remains unclear. Methods By using a serum-free neural induction …

Simple and efficient derivation of mouse embryonic stem cell lines using differentiation inhibitors or proliferation stimulators

KH Lee, CK Chuang, SF Guo, CF Tu - Stem cells and development, 2012 - liebertpub.com
The inhibition of endogenous differentiation-inducing signaling or the enhancement of
growth capacity and viability of preimplantation embryos, via 2i (PD0325901 and …

Contribution of mouse embryonic stem cells and induced pluripotent stem cells to chimeras through injection and coculture of embryos

J Guo, B Wu, S Li, S Bao, L Zhao, S Hu… - Stem cells …, 2014 - Wiley Online Library
Blastocyst injection and morula aggregation are commonly used to evaluate stem cell
pluripotency based on chimeric contribution of the stem cells. To assess the protocols for …

[HTML][HTML] Enhancement of insulin-producing cell differentiation from embryonic stem cells using pax4-nucleofection method

HT Lin, CL Kao, KH Lee, YL Chang… - World journal of …, 2007 - ncbi.nlm.nih.gov
AIM: To enhance the differentiation of insulin producing cell (IPC) ability from embryonic
stem (ES) cells in vitro. METHODS: Four-day embryoid body (EB)-formatted ES cells were …

Collagen IV significantly enhances migration and transplantation of embryonic stem cells: involvement of α2β1 integrin-mediated actin remodeling

HY Li, CY Liao, KH Lee, HC Chang… - Cell …, 2011 - journals.sagepub.com
Embryonic stem (ES) cell transplantation represents a potential means for the treatment of
degenerative diseases and injuries. As appropriate distribution of transplanted ES cells in …

An efficient and mass reproducible method for vitrifying mouse embryos on a paper in cryotubes

KH Lee, JC Sun, C Chuang, SF Guo, CF Tu, JC Ju - Cryobiology, 2013 - Elsevier
To date, the most successful and popular vitrification method is based on the minimum
volume cooling (MVC) concept, in which embryos are vitrified in a very small volume of …