[HTML][HTML] Aquatic environmental DNA: A review of the macro-organismal biomonitoring revolution
Environmental DNA (eDNA) is the fastest growing biomonitoring tool fuelled by two key
features: time efficiency and sensitivity. Technological advancements allow rapid …
features: time efficiency and sensitivity. Technological advancements allow rapid …
MiFish metabarcoding: a high-throughput approach for simultaneous detection of multiple fish species from environmental DNA and other samples
We reviewed the current methodology and practices of the DNA metabarcoding approach
using a universal PCR primer pair MiFish, which co-amplifies a short fragment of fish DNA …
using a universal PCR primer pair MiFish, which co-amplifies a short fragment of fish DNA …
Fishing for fish environmental DNA: Ecological applications, methodological considerations, surveying designs, and ways forward
Vast global declines of freshwater and marine fish diversity and population abundance pose
serious threats to both ecosystem sustainability and human livelihoods. Environmental DNA …
serious threats to both ecosystem sustainability and human livelihoods. Environmental DNA …
Improving metabarcoding taxonomic assignment: A case study of fishes in a large marine ecosystem
DNA metabarcoding is an important tool for molecular ecology. However, its effectiveness
hinges on the quality of reference sequence databases and classification parameters …
hinges on the quality of reference sequence databases and classification parameters …
Comparing eDNA metabarcoding primers for assessing fish communities in a biodiverse estuary
Metabarcoding of environmental DNA is increasingly used for biodiversity assessments in
aquatic communities. The efficiency and outcome of these efforts are dependent upon either …
aquatic communities. The efficiency and outcome of these efforts are dependent upon either …
The elephant in the lab (and field): Contamination in aquatic environmental DNA studies
AJ Sepulveda, PR Hutchins, M Forstchen… - Frontiers in Ecology …, 2020 - frontiersin.org
The rapid evolution of environmental (e) DNA methods has resulted in knowledge gaps in
smaller, yet critical details like proper use of negative controls to detect contamination …
smaller, yet critical details like proper use of negative controls to detect contamination …
What do you mean by false positive?
Misunderstandings regarding the term “false positive” present a significant hurdle to broad
adoption of eDNA monitoring methods. Here, we identify three challenges to clear …
adoption of eDNA monitoring methods. Here, we identify three challenges to clear …
Making environmental DNA (eDNA) biodiversity records globally accessible
Environmental DNA (eDNA) metabarcoding, where specific DNA markers (barcodes) are
purified from the environment, sequenced, and used to identify organisms, is revolutionizing …
purified from the environment, sequenced, and used to identify organisms, is revolutionizing …
[HTML][HTML] Strategic considerations for invasive species managers in the utilization of environmental DNA (eDNA): steps for incorporating this powerful surveillance tool
J Morisette, S Burgiel, K Brantley… - … journal of applied …, 2021 - ncbi.nlm.nih.gov
Invasive species surveillance programs can utilize environmental DNA sampling and
analysis to provide information on the presence of invasive species. Wider utilization of …
analysis to provide information on the presence of invasive species. Wider utilization of …
Best practice guidelines for environmental DNA biomonitoring in Australia and New Zealand
Environmental DNA (eDNA)‐based methods are increasingly used by government agencies
to detect pests and threatened species, and for broader biodiversity monitoring. Given rapid …
to detect pests and threatened species, and for broader biodiversity monitoring. Given rapid …