Nearly all infectious agents contain DNA or RNA genomes, making sequencing an attractive
approach for pathogen detection. The cost of high-throughput or next-generation …

Agnostic metagenomic next-generation sequencing (mNGS) has emerged as a promising
single, universal pathogen detection method for infectious disease diagnostics. This …

Background Metagenomic next-generation sequencing (NGS) of cerebrospinal fluid (CSF)
has the potential to identify a broad range of pathogens in a single test. Methods In a 1-year …

The application of sequencing technology is shifting from research to clinical laboratories
owing to rapid technological developments and substantially reduced costs. However …

Importance Identifying infectious causes of subacute or chronic meningitis can be
challenging. Enhanced, unbiased diagnostic approaches are needed. Objective To present …

Over the past two decades, the diagnosis rate for patients with encephalitis has remained
poor despite advances in pathogen-specific testing such as PCR and antigen assays …

Metagenomic shotgun sequencing has the potential to transform how serious infections are
diagnosed by offering universal, culture-free pathogen detection. This may be especially …

Background: Tuberculosis (TB) is now the leading cause of death from infectious disease.
Rapid screening and diagnostic methods for TB are urgently required. Rapid development …

INTRODUCTION With established applications of next-generation sequencing in inherited
diseases and oncology, clinical laboratories are evaluating the use of metagenomics for …

Background Accurate etiology diagnosis is crucial for central nervous system infections
(CNS infections). The diagnostic value of metagenomic next-generation sequencing …