l-arginine production in Corynebacterium glutamicum: manipulation and optimization of the metabolic process

Y Jiang, Q Sheng, XY Wu, BC Ye… - Critical Reviews in …, 2021 - Taylor & Francis
As an important semi-essential amino acid, l-arginine is extensively used in the food and
pharmaceutical fields. At present, l-arginine production depends on cost-effective, green …

Modular pathway engineering of Corynebacterium glutamicum for production of the glutamate-derived compounds ornithine, proline, putrescine, citrulline, and …

JVK Jensen, D Eberhardt, VF Wendisch - Journal of Biotechnology, 2015 - Elsevier
The glutamate-derived bioproducts ornithine, citrulline, proline, putrescine, and arginine
have applications in the food and feed, cosmetic, pharmaceutical, and chemical industries …

Recent advances in amino acid production

M Ikeda, S Takeno - Corynebacterium Glutamicum: Biology and …, 2020 - Springer
The annual world production of amino acids is currently estimated at more than seven
million tons and is expected to reach ten million tons by 2022. This giant market has been …

Engineering Thermoanaerobacterium aotearoense SCUT27 with argR knockout for enhanced ethanol production from lignocellulosic hydrolysates

C Qu, L Chen, H Fu, J Wang - Bioresource technology, 2020 - Elsevier
Abstract Although Thermoanaerobacterium aotearoense SCUT27 (SCUT27) could co-utilize
glucose and xylose, the presence of glucose still repressed xylose catabolism. Arginine …

[HTML][HTML] High-level production of ornithine by expression of the feedback inhibition-insensitive N-acetyl glutamate kinase in the sake yeast Saccharomyces cerevisiae

M Ohashi, R Nasuno, S Isogai, H Takagi - Metabolic Engineering, 2020 - Elsevier
We previously reported that intracellular proline (Pro) confers tolerance to ethanol on the
yeast Saccharomyces cerevisiae. In this study, to improve the ethanol productivity of sake, a …

Reengineering of the feedback-inhibition enzyme N-acetyl-l-glutamate kinase to enhance l-arginine production in Corynebacterium crenatum

J Zhang, M Xu, X Ge, X Zhang, T Yang… - Journal of Industrial …, 2017 - academic.oup.com
N-acetyl-l-glutamate kinase (NAGK) catalyzes the second step of l-arginine biosynthesis and
is inhibited by l-arginine in Corynebacterium crenatum. To ascertain the basis for the …

Functional Analysis of Feedback Inhibition-Insensitive Variants of N-Acetyl Glutamate Kinase Found in Sake Yeast Mutants with Ornithine Overproduction

M Ohashi, S Isogai, H Takagi - Microbiology spectrum, 2022 - Am Soc Microbiol
In the yeast Saccharomyces cerevisiae, N-acetyl glutamate kinase (NAGK), which catalyzes
the phosphorylation of N-acetyl glutamate to form N-acetyl glutamyl-5-phosphate, is one of …

Monomeric Corynebacterium glutamicum N-acetyl glutamate kinase maintains sensitivity to L-arginine but has a lower intrinsic catalytic activity

Y Huang, C Li, H Zhang, S Liang, S Han, Y Lin… - Applied microbiology …, 2016 - Springer
N-acetyl glutamate kinase (NAGK) is a key enzyme in the synthesis of L-arginine, and L-
arginine-sensitive NAGK typically has hexameric architecture. Defining the relationship …

Conformational dynamics play important roles upon the function of N-acetylglutamate kinase

X Yang - Applied microbiology and biotechnology, 2017 - Springer
N-acetylglutamate kinase (NAGK) catalyzes the phosphorylation of N-acetylglutamate. In
many bacteria, NAGK catalysis is the rate controlling step in the L-arginine biosynthesis …

Engineering of N-acetyl-l-glutamate kinase from Corynebacterium glutamicum toward improved catalytic efficiency and thermostability

J Zhang, M Xu, X Zhang, T Yang, Z Xu, Z Rao - Journal of Molecular …, 2016 - Elsevier
N-Acetyl-L-glutamate kinase (NAGK) is a key enzyme in the synthesis of L-arginine.
Although, solving the problem of L-arginine feedback inhibition for NAGK is a vital way for …