The technological landscape and applications of single-cell multi-omics

A Baysoy, Z Bai, R Satija, R Fan - Nature Reviews Molecular Cell …, 2023 - nature.com
Single-cell multi-omics technologies and methods characterize cell states and activities by
simultaneously integrating various single-modality omics methods that profile the …

Single-cell proteomics enabled by next-generation sequencing or mass spectrometry

HM Bennett, W Stephenson, CM Rose, S Darmanis - Nature Methods, 2023 - nature.com
In the last decade, single-cell RNA sequencing routinely performed on large numbers of
single cells has greatly advanced our understanding of the underlying heterogeneity of …

mRNAs, proteins and the emerging principles of gene expression control

C Buccitelli, M Selbach - Nature Reviews Genetics, 2020 - nature.com
Gene expression involves transcription, translation and the turnover of mRNAs and proteins.
The degree to which protein abundances scale with mRNA levels and the implications in …

Single-cell multiomics: technologies and data analysis methods

J Lee, DY Hyeon, D Hwang - Experimental & Molecular Medicine, 2020 - nature.com
Advances in single-cell isolation and barcoding technologies offer unprecedented
opportunities to profile DNA, mRNA, and proteins at a single-cell resolution. Recently, bulk …

Artificial intelligence for the prevention and clinical management of hepatocellular carcinoma

J Calderaro, TP Seraphin, T Luedde, TG Simon - Journal of hepatology, 2022 - Elsevier
Hepatocellular carcinoma (HCC) currently represents the fifth most common malignancy and
the third-leading cause of cancer-related death worldwide, with incidence and mortality rates …

Integrative single-cell analysis

T Stuart, R Satija - Nature reviews genetics, 2019 - nature.com
The recent maturation of single-cell RNA sequencing (scRNA-seq) technologies has
coincided with transformative new methods to profile genetic, epigenetic, spatial, proteomic …

Applications of multi‐omics analysis in human diseases

C Chen, J Wang, D Pan, X Wang, Y Xu, J Yan… - MedComm, 2023 - Wiley Online Library
Multi‐omics usually refers to the crossover application of multiple high‐throughput screening
technologies represented by genomics, transcriptomics, single‐cell transcriptomics …

Simultaneous epitope and transcriptome measurement in single cells

M Stoeckius, C Hafemeister, W Stephenson… - Nature …, 2017 - nature.com
High-throughput single-cell RNA sequencing has transformed our understanding of complex
cell populations, but it does not provide phenotypic information such as cell-surface protein …

Single-cell RNA sequencing to explore immune cell heterogeneity

E Papalexi, R Satija - Nature Reviews Immunology, 2018 - nature.com
Advances in single-cell RNA sequencing (scRNA-seq) have allowed for comprehensive
analysis of the immune system. In this Review, we briefly describe the available scRNA-seq …

Multiplexed quantification of proteins and transcripts in single cells

VM Peterson, KX Zhang, N Kumar, J Wong, L Li… - Nature …, 2017 - nature.com
We present a tool to measure gene and protein expression levels in single cells with DNA-
labeled antibodies and droplet microfluidics. Using the RNA expression and protein …