Poly (ethylene oxide) poly (propylene oxide) poly (ethylene oxide) block copolymer surfactants in aqueous solutions and at interfaces: thermodynamics, structure …

P Alexandridis, TA Hatton - Colloids and Surfaces A: Physicochemical and …, 1995 - Elsevier
The association properties of poly (ethylene oxide)-block-poly (propyleneoxide)-block-poly
(ethylene oxide)(PEO PPO PEO) copolymers (commercially available as Poloxamers …

Mammalian cell protein expression for biopharmaceutical production

J Zhu - Biotechnology advances, 2012 - Elsevier
Mammalian cell expression has become the dominant recombinant protein production
system for clinical applications because of its capacity for post-translational modification and …

[图书][B] Encyclopedia of surface and colloid science

P Somasundaran - 2006 - books.google.com
The frontier of surface and colloid science has moved to smaller and smaller regions and
deeper and deeper into the living world. It is a privilege to introduce the second edition of the …

Animal-cell damage in sparged bioreactors

Y Chisti - Trends in biotechnology, 2000 - cell.com
The gas sparging of culture broth causes damage to suspended animal cells. However,
despite this, sparged bioreactors remain the preferred means of cell culture because …

Integration of surface modification and 3D fabrication techniques to prepare patterned poly (L-lactide) substrates allowing regionally selective cell adhesion

A Park, B Wu, LG Griffith - Journal of Biomaterials Science, Polymer …, 1998 - Taylor & Francis
Regeneration of organizationally complex tissue requires regulation of spatial distributions
of particular cell types in three dimensions. In this paper we demonstrate an integration of …

The potential of hydrodynamic damage to animal cells of industrial relevance: current understanding

W Hu, C Berdugo, JJ Chalmers - Cytotechnology, 2011 - Springer
Suspension animal cell culture is now routinely scaled up to bioreactors on the order of
10,000 L, and greater, to meet commercial demand. However, the concern of the 'shear …

Sparged animal cell bioreactors: mechanism of cell damage and Pluronic F‐68 protection

DW Murhammer, CF Goochee - Biotechnology progress, 1990 - Wiley Online Library
Pluronic F‐68 is a widely used protective agent in sparged animal cell bioreactors. In this
study, the attachment‐independent Spodoptera frugiperda Sf9 insect cell line was used to …

Shear sensitivity

Y Chisti - Encyclopedia of industrial biotechnology: bioprocess …, 2009 - Wiley Online Library
Susceptibility to hydrodynamic and mechanical shear forces affects performance of cultured
cells of animals, plants, microalgae, and cyanobacteria. In addition, hydromechanical forces …

Role of hydrodynamic shear in the cultivation of animal, plant and microbial cells

JB Joshi, CB Elias, MS Patole - The Chemical Engineering Journal and the …, 1996 - Elsevier
The rapid developments in biotechnology have resulted in the identification and use of a
large variety of biologically active substances produced from microbial, plant and animal …

Upstream processes in antibody production: evaluation of critical parameters

E Jain, A Kumar - Biotechnology advances, 2008 - Elsevier
The demand for monoclonal antibody for therapeutic and diagnostic applications is rising
constantly which puts up a need to bring down the cost of its production. In this context it …