A comparative overview of epigenomic profiling methods
In the past decade, assays that profile different aspects of the epigenome have grown
exponentially in number and variation. However, standard guidelines for researchers to …
exponentially in number and variation. However, standard guidelines for researchers to …
The evidence for functional non-CpG methylation in mammalian cells
In mammalian genomes, the methylation of cytosine residues within CpG dinucleotides is
crucial to normal development and cell differentiation. However, methylation of cytosines in …
crucial to normal development and cell differentiation. However, methylation of cytosines in …
Critical evaluation of the Illumina MethylationEPIC BeadChip microarray for whole-genome DNA methylation profiling
Background In recent years the Illumina HumanMethylation450 (HM450) BeadChip has
provided a user-friendly platform to profile DNA methylation in human samples. However …
provided a user-friendly platform to profile DNA methylation in human samples. However …
Direct detection of DNA methylation during single-molecule, real-time sequencing
BA Flusberg, DR Webster, JH Lee, KJ Travers… - Nature …, 2010 - nature.com
We describe the direct detection of DNA methylation, without bisulfite conversion, through
single-molecule, real-time (SMRT) sequencing. In SMRT sequencing, DNA polymerases …
single-molecule, real-time (SMRT) sequencing. In SMRT sequencing, DNA polymerases …
The behaviour of 5-hydroxymethylcytosine in bisulfite sequencing
Background We recently showed that enzymes of the TET family convert 5-mC to 5-
hydroxymethylcytosine (5-hmC) in DNA. 5-hmC is present at high levels in embryonic stem …
hydroxymethylcytosine (5-hmC) in DNA. 5-hmC is present at high levels in embryonic stem …
Methylation determines fibroblast activation and fibrogenesis in the kidney
W Bechtel, S McGoohan, EM Zeisberg, GA Müller… - Nature medicine, 2010 - nature.com
Fibrogenesis is a pathological wound repair process that fails to cease, even when the initial
insult has been removed. Fibroblasts are principal mediators of fibrosis, and fibroblasts from …
insult has been removed. Fibroblasts are principal mediators of fibrosis, and fibroblasts from …
Quantitative comparison of DNA methylation assays for biomarker development and clinical applications
Nature biotechnology, 2016 - nature.com
DNA methylation patterns are altered in numerous diseases and often correlate with
clinically relevant information such as disease subtypes, prognosis and drug response. With …
clinically relevant information such as disease subtypes, prognosis and drug response. With …
Epigenetic transgenerational actions of vinclozolin on promoter regions of the sperm epigenome
Previous observations have demonstrated that embryonic exposure to the endocrine
disruptor vinclozolin during gonadal sex determination promotes transgenerational adult …
disruptor vinclozolin during gonadal sex determination promotes transgenerational adult …
Effects of cytosine methylation on transcription factor binding sites
Background DNA methylation in promoters is closely linked to downstream gene repression.
However, whether DNA methylation is a cause or a consequence of gene repression …
However, whether DNA methylation is a cause or a consequence of gene repression …
Examination of the specificity of DNA methylation profiling techniques towards 5-methylcytosine and 5-hydroxymethylcytosine
SG Jin, S Kadam, GP Pfeifer - Nucleic acids research, 2010 - academic.oup.com
ABSTRACT DNA cytosine-5 methylation is a well-studied epigenetic pathway implicated in
gene expression control and disease pathogenesis. Different technologies have been …
gene expression control and disease pathogenesis. Different technologies have been …