The development of single-cell and spatial transcriptomics methods was instrumental in the
conception of the Human Cell Atlas initiative, which aims to generate an integrated map of …

Germinal center (GC) B cells are the source of the high-affinity, class-switched antibodies
required for protective immunity. The unique biology of GC B cells involves iterative rounds …

Despite their crucial role in health and disease, our knowledge of immune cells within
human tissues remains limited. We surveyed the immune compartment of 16 tissues from 12 …

Spatial transcriptomic technologies promise to resolve cellular wiring diagrams of tissues in
health and disease, but comprehensive mapping of cell types in situ remains a challenge …

The presence of intratumoral tertiary lymphoid structures (TLS) is associated with positive
clinical outcomes and responses to immunotherapy in cancer. Here, we used spatial …

Cellular function in tissue is dependent on the local environment, requiring new methods for
spatial mapping of biomolecules and cells in the tissue context. The emergence of spatial …

Germinal centres (GC) are lymphoid structures in which B cells acquire affinity-enhancing
somatic hypermutations (SHM), with surviving clones differentiating into memory B cells …

Spatial transcriptomics technologies generate gene expression profiles with spatial context,
requiring spatially informed analysis tools for three key tasks, spatial clustering, multisample …

In this study, we extended co-indexing of transcriptomes and epitopes (CITE) to the spatial
dimension and demonstrated high-plex protein and whole transcriptome co-mapping. We …

Biological heterogeneity in diffuse large B cell lymphoma (DLBCL) is partly driven by cell-of-
origin subtypes and associated genomic lesions, but also by diverse cell types and cell …