Photochemical mechanisms of fluorophores employed in single‐molecule localization microscopy

K Kikuchi, LD Adair, J Lin, EJ New… - Angewandte Chemie, 2023 - Wiley Online Library
Decoding cellular processes requires visualization of the spatial distribution and dynamic
interactions of biomolecules. It is therefore not surprising that innovations in imaging …

Recent advances in the development of fluorescent probes for γ‐glutamyltranspeptidase

ZY Hu, XY Chen, YS Yang, SJ Wang, ZG Hu… - Coordination Chemistry …, 2024 - Elsevier
Abstract γ-Glutamyltranspeptidase (GGT) plays a significant role in the regulation of cellular
redox homeostasis and other physiological or pathophysiological processes. The over …

Method to diversify cyanine chromophore functionality enables improved biomolecule tracking and intracellular imaging

SM Usama, SC Marker, DH Li… - Journal of the …, 2023 - ACS Publications
Heptamethine indocyanines are invaluable probes for near-infrared (NIR) imaging. Despite
broad use, there are only a few synthetic methods to assemble these molecules, and each …

The DNA-PAINT palette: a comprehensive performance analysis of fluorescent dyes

PR Steen, EM Unterauer, LA Masullo, J Kwon… - Nature …, 2024 - nature.com
DNA points accumulation for imaging in nanoscale topography (DNA-PAINT) is a super-
resolution fluorescence microscopy technique that achieves single-molecule 'blinking'by …

Heptamethine cyanine dyes with ultra‐efficient excited‐state nonradiative decay for synergistic photothermal immunotherapy

X Xia, C Shi, S He, R Wang, Z Zhang… - Advanced Functional …, 2023 - Wiley Online Library
Organic dyes in the excited singlet state (S1) decay via intersystem crossing (ISC) to the
triplet state, radiative dissipation as fluorescence, or nonradiative decay for thermal …

Small Fluorogenic Amino Acids for Peptide‐Guided Background‐Free Imaging

F De Moliner, Z Konieczna… - Angewandte Chemie …, 2023 - Wiley Online Library
The multiple applications of super‐resolution microscopy have prompted the need for
minimally invasive labeling strategies for peptide‐guided fluorescence imaging. Many …

General strategy to improve the photon budget of thiol-conjugated cyanine dyes

Y Zhang, C Yang, S Peng, J Ling, P Chen… - Journal of the …, 2023 - ACS Publications
Maleimide-cysteine chemistry has been a routine practice for the site-specific labeling of
fluorophores to proteins since the 1950s. This approach, however, cannot bring out the best …

Cyanine phototruncation enables spatiotemporal cell labeling

H Fukushima, SS Matikonda, SM Usama… - Journal of the …, 2022 - ACS Publications
Photoconvertible tracking strategies assess the dynamic migration of cell populations. Here
we develop p hototruncation-a ssisted c ell t racking (PACT) and apply it to evaluate the …

Isotropic three-dimensional dual-color super-resolution microscopy with metal-induced energy transfer

JC Thiele, M Jungblut, DA Helmerich, R Tsukanov… - Science …, 2022 - science.org
Over the past two decades, super-resolution microscopy has seen a tremendous
development in speed and resolution, but for most of its methods, there exists a remarkable …

Aryl‐Modified Pentamethyl Cyanine Dyes at the C2'Position: A Tunable Platform for Activatable Photosensitizers

F Han, SA Abbas Abedi, S He, H Zhang… - Advanced …, 2024 - Wiley Online Library
Pentamethyl cyanine dyes are promising fluorophores for fluorescence sensing and
imaging. However, advanced biomedical applications require enhanced control of their …