A MutS Pichia pastoris strain that had been genetically modified to produce and secrete sea
raven antifreeze protein was used as a model system to demonstrate the implementation of …

This paper reports development and implementation of superior fermentation strategies for β-
galactosidase production by Lactobacillus acidophilus in a stirred-tank bioreactor. Process …

A mathematical model was formulated to simulate cell growth, plasmid loss and recombinant
protein production during the aerobic culture of a recombinant yeast S. cerevisiae. Model …

Overexpression of the homologous protein proteinase A (PrA) in Saccharomyces cerevisiae
has been achieved by inserting the PrA gene (PEP4) with its own promoter on a 2µ …

A recombinant Saccharomyces cerevisiae expressing β-galactosidase under the control of
the GAL10 promoter was constructed. The strain was used as a model to study fuzzy control …

To produce a recombinant protein, it is critically important to optimize and control
bioprocesses based on knowledge of a cell's genetic, metabolic, and kinetic behavior. It is …

The Escherichia coli beta-galactosidase (EC 3.2. 1.23) expressed intracellularly as soluble,
biologically active enzyme in the yeast Saccharomyces cerevisiae was recovered from …

Optimisation of the glucose feed rate and feed concentration for the production of a
recombinant protein (β-galactosidase) with respect to two different objective functions is …

In order to describe and predict the growth and expression of recombinant proteins by using
a genetically modified Pichia pastoris, we developed a number of unstructured models …

A simple, structured model was developed to describe the growth and product formation
behavior of two recombinant strains of Saccharomyces cerevisiae (JG176 and JG180), both …