Creating new fluorescent probes for cell biology
Fluorescent probes are one of the cornerstones of real-time imaging of live cells and a
powerful tool for cell biologists. They provide high sensitivity and great versatility while …
powerful tool for cell biologists. They provide high sensitivity and great versatility while …
Bimolecular fluorescence complementation (BiFC) analysis as a probe of protein interactions in living cells
TK Kerppola - Annu. Rev. Biophys., 2008 - annualreviews.org
Protein interactions are a fundamental mechanism for the generation of biological regulatory
specificity. The study of protein interactions in living cells is of particular significance …
specificity. The study of protein interactions in living cells is of particular significance …
Imaging biochemistry inside cells
FS Wouters, PJ Verveer, PIH Bastiaens - Trends in cell biology, 2001 - cell.com
Proteins provide the building blocks for multicomponent molecular units, or pathways, from
which higher cellular functions emerge. These units consist of either assemblies of …
which higher cellular functions emerge. These units consist of either assemblies of …
FHL2 anchors mitochondria to actin and adapts mitochondrial dynamics to glucose supply
Mitochondrial movement and distribution are fundamental to their function. Here we report a
mechanism that regulates mitochondrial movement by anchoring mitochondria to the F-actin …
mechanism that regulates mitochondrial movement by anchoring mitochondria to the F-actin …
The multifunctional roles of the four-and-a-half-LIM only protein FHL2
M Johannessen, S Møller, T Hansen, U Moens… - Cellular and Molecular …, 2006 - Springer
Numerous cellular processes require the concerted action of multiple proteins that assemble
in functional complexes. Protein-protein interaction domains allow specific proteins to …
in functional complexes. Protein-protein interaction domains allow specific proteins to …
Subcellular targeting of metabolic enzymes to titin in heart muscle may be mediated by DRAL/FHL-2
S Lange, D Auerbach, P McLoughlin… - Journal of cell …, 2002 - journals.biologists.com
During sarcomere contraction skeletal and cardiac muscle cells consume large amounts of
energy. To satisfy this demand, metabolic enzymes are associated with distinct regions of …
energy. To satisfy this demand, metabolic enzymes are associated with distinct regions of …
[HTML][HTML] The transcriptional coactivator FHL2 transmits Rho signals from the cell membrane into the nucleus
JM Müller, E Metzger, H Greschik, AK Bosserhoff… - The EMBO …, 2002 - embopress.org
GTPases of the Rho family are transducers of extracellular signals and control cellular
processes such as organization of the actin cytoskeleton, motility, adhesion and gene …
processes such as organization of the actin cytoskeleton, motility, adhesion and gene …
Bimolecular fluorescence complementation: visualization of molecular interactions in living cells
TK Kerppola - Methods in cell biology, 2008 - Elsevier
A variety of experimental methods have been developed for the analysis of protein
interactions. The majority of these methods either require disruption of the cells to detect …
interactions. The majority of these methods either require disruption of the cells to detect …
Dynamic gene expression profiling using a microfabricated living cell array
We describe the development of a microfluidic platform for continuous monitoring of gene
expression in live cells. This optically transparent microfluidic device integrates high …
expression in live cells. This optically transparent microfluidic device integrates high …
Insulin-like growth factor-binding protein 5 (IGFBP-5) interacts with a four and a half LIM protein 2 (FHL2)
YG Amaar, GR Thompson, TA Linkhart, ST Chen… - Journal of Biological …, 2002 - ASBMB
Recent studies using insulin-like growth factor I (IGF-I) knockout mice demonstrate that IGF-
binding protein (IGFBP)-5, an important bone formation regulator, itself is a growth factor …
binding protein (IGFBP)-5, an important bone formation regulator, itself is a growth factor …