Protein engineering: advances in phage display for basic science and medical research

EK Davydova - Biochemistry (Moscow), 2022 - Springer
Abstract Functional Protein Engineering became the hallmark in biomolecule manipulation
in the new millennium, building on and surpassing the underlying structural DNA …

Photophysical Properties of Fluorescent Labels: A Meta‐Analysis to Guide Probe Selection Amidst Challenges with Available Data

D Cavazos‐Elizondo, A Aguirre‐Soto - Analysis & Sensing, 2022 - Wiley Online Library
Fluorescent labels have been paramount in the advancement of molecular biology and
related fields, for they unveil Nature's unknowns. Numerous labels have been developed …

A rapid simple point-of-care assay for the detection of SARS-CoV-2 neutralizing antibodies

P Kongsuphol, H Jia, HL Cheng, Y Gu… - Communications …, 2021 - nature.com
Abstract Background Neutralizing antibodies (NAbs) prevent pathogens from infecting host
cells. Detection of SARS-CoV-2 NAbs is critical to evaluate herd immunity and monitor …

Developing a SARS-CoV-2 antigen test using engineered affinity proteins

S Kim, E Yee, EA Miller, Y Hao, DMY Tay… - … Applied Materials & …, 2021 - ACS Publications
The ongoing COVID-19 pandemic has clearly established how vital rapid, widely accessible
diagnostic tests are in controlling infectious diseases and how difficult and slow it is to scale …

Chemical diversification of simple synthetic antibodies

M Islam, HP Kehoe, JB Lissoos, M Huang… - ACS chemical …, 2021 - ACS Publications
Antibodies possess properties that make them valuable as therapeutics, diagnostics, and
basic research tools. However, antibody chemical reactivity and covalent antigen binding …

Modular Design for Proteins Assembling into Antifouling Coatings: Case of Gold Surfaces

C Zheng, N Alvisi, RJ de Haas, Z Zhang, H Zuilhof… - Langmuir, 2023 - ACS Publications
We analyze modularity for a BME triblock protein designed to self-assemble into antifouling
coatings. Previously, we have shown that the design performs well on silica surfaces when B …

Development of a cellulose-based 96-well plate vertical flow pull-down assay

HL Cheng, H Jia, SM Lim, SY Ng, P Kongsuphol… - Analytical …, 2023 - pubs.rsc.org
The abundance and low production cost of biomaterial cellulose paper have attracted
attention for many applications. Point-of-care (PoC) diagnostic tests have been successfully …

Accelerating the optimization of vertical flow assay performance guided by a rational systematic model-based approach

DMY Tay, S Kim, Y Hao, EH Yee, H Jia… - Biosensors and …, 2023 - Elsevier
Rapid diagnostic tests (RDTs) have shown to be instrumental in healthcare and disease
control. However, they have been plagued by many inefficiencies in the laborious empirical …

Optical Detection of Interleukin-6 Using Liquid Janus Emulsions Using Hyperthermophilic Affinity Proteins

M Chen, EI Corless, BP Engelward, TM Swager - ACS omega, 2024 - ACS Publications
When equal volumes of two immiscible liquids are mixed (eg, a hydrocarbon and a
fluorocarbon), Janus droplets can form in an aqueous solution. In a gravity-aligned Janus …

Functional comparison of paper-based immunoassays based on antibodies and engineered binding proteins

KJ Sung, YJ Al Maalouf, QR Johns, EA Miller, HD Sikes - Analyst, 2020 - pubs.rsc.org
Binding protein scaffolds, such as rcSso7d, have been investigated for use in diagnostic
tests; however, the functional performance of rcSso7d has not yet been studied in …