RNA sequencing (RNAseq) can reveal gene fusions, splicing variants, mutations/indels in
addition to differential gene expression, thus providing a more complete genetic picture than …

Organotypic models of patient-specific tumours are revolutionizing our understanding of
cancer heterogeneity and its implications for personalized medicine. These advancements …

Liver metastasis, the leading cause of colorectal cancer mortality, exhibits a highly
heterogeneous and suppressive immune microenvironment. Here, we sequenced 97 …

The development of neoplasia involves a complex and continuous interplay between
malignantly transformed cells and the tumour microenvironment (TME). Cancer …

Colorectal cancer has served as a genetic and biological paradigm for the evolution of solid
tumors, and these insights have illuminated early detection, risk stratification, prevention …

Adult stem cell–based organoid technology is a versatile tool for the generation and long-
term maintenance of near-native 3D epithelial tissues in vitro. The generation of cancer …

Single-cell analysis is a valuable tool for dissecting cellular heterogeneity in complex
systems. However, a comprehensive single-cell atlas has not been achieved for humans …

Simple Summary Cell cultures can be carried out in three dimensions (3D). Organoids and
spheroids are different 3D cell culture models that can be cultured with different techniques …

Cancer represents an evolutionary process through which growing malignant populations
genetically diversify, leading to tumour progression, relapse and resistance to therapy. In …

Hepatocellular carcinoma (HCC), the most common form of primary liver cancer, typically
develops on the background of chronic liver disease and is an aggressive disease with …