Although the roots of Ras sprouted from the rich history of retrovirus research, it was the
discovery of mutationally activated RAS genes in human cancer in 1982 that stimulated an …

Small GTP-binding proteins (G proteins) exist in eukaryotes from yeast to human and
constitute a superfamily consisting of more than 100 members. This superfamily is …

The ADP-ribosylation factor (ARF) small GTPases regulate vesicular traffic and organelle
structure by recruiting coat proteins, regulating phospholipid metabolism and modulating the …

In vitro synthesis of endoplasmic reticulum-derived transpofl vesicles has been reconstituted
with washed membranes and three soluble proteins (Sarl p, Secl3p complex, and Sec23p …

THE definition of cellular organelles has evolved over the last hundred years largely driven
by morphologic observations, but more recently has been supplemented and complemented …

The small GTP-binding protein rab5 was previously localized on early endosomes and on
the cytoplasmic face of the plasma membrane. Using a cell-free assay, we have now tested …

Recent advances have uncovered the general protein apparatus used by all eukaryotes for
intracellular transport, including secretion and endocytosis, and for triggered exocytosis of …

A WIDE variety of membrane transformations important in intracellular transport are inhibited
by the fungal metabolite brefeldin A (refs 1–4), implying that the target for this drug is central …

Eukaryotic life depends on the spatial and temporal organization of cellular membrane
systems. Recent advances in understanding the machinery of vesicle transport have …

THE fungal metabolite brefeldin A is a powerful tool for investigating membrane traffic in
eukaryotic cells1. The effects of brefeldin A on traffic are partly explained by its ability to …