Whole-body and whole-organ clearing and imaging techniques with single-cell resolution: toward organism-level systems biology in mammals

EA Susaki, HR Ueda - Cell chemical biology, 2016 - cell.com
Organism-level systems biology aims to identify, analyze, control and design cellular circuits
in organisms. Many experimental and computational approaches have been developed …

Measurement of tissue optical properties in the context of tissue optical clearing

AN Bashkatov, KV Berezin… - … of biomedical optics, 2018 - spiedigitallibrary.org
Nowadays, dynamically developing optical (photonic) technologies play an ever-increasing
role in medicine. Their adequate and effective implementation in diagnostics, surgery, and …

Shrinkage-mediated imaging of entire organs and organisms using uDISCO

C Pan, R Cai, FP Quacquarelli, A Ghasemigharagoz… - Nature …, 2016 - nature.com
Recent tissue-clearing approaches have become important alternatives to standard
histology approaches. However, light scattering in thick tissues and the size restrictions on …

Whole-body tissue stabilization and selective extractions via tissue-hydrogel hybrids for high-resolution intact circuit mapping and phenotyping

JB Treweek, KY Chan, NC Flytzanis, B Yang… - Nature protocols, 2015 - nature.com
To facilitate fine-scale phenotyping of whole specimens, we describe here a set of tissue
fixation-embedding, detergent-clearing and staining protocols that can be used to transform …

Transcranial infrared laser stimulation produces beneficial cognitive and emotional effects in humans

DW Barrett, F Gonzalez-Lima - Neuroscience, 2013 - Elsevier
This is the first controlled study demonstrating the beneficial effects of transcranial laser
stimulation on cognitive and emotional functions in humans. Photobiomodulation with red to …

A guidebook for DISCO tissue clearing

M Molbay, ZI Kolabas, MI Todorov, TL Ohn… - Molecular Systems …, 2021 - embopress.org
Histological analysis of biological tissues by mechanical sectioning is significantly time‐
consuming and error‐prone due to loss of important information during sample slicing. In the …

Bone CLARITY: Clearing, imaging, and computational analysis of osteoprogenitors within intact bone marrow

A Greenbaum, KY Chan, T Dobreva, D Brown… - Science translational …, 2017 - science.org
Bone tissue harbors unique and essential physiological processes, such as hematopoiesis,
bone growth, and bone remodeling. To enable visualization of these processes at the …

Spectroscopic photoacoustic imaging of lipid-rich plaques in the human aorta in the 740 to 1400 nm wavelength range

TJ Allen, A Hall, AP Dhillon, JS Owen… - Journal of biomedical …, 2012 - spiedigitallibrary.org
Spectroscopic photoacoustic imaging has the potential to discriminate between normal and
lipid-rich atheromatous areas of arterial tissue by exploiting the differences in the absorption …

Tissue optical immersion clearing

EA Genina, AN Bashkatov… - Expert review of medical …, 2010 - Taylor & Francis
In this article, we discuss the optical immersion method based on refractive index matching
of scatterers (eg, collagen, elastin fibers, cells and cell compartments) and the ground …

Recent progress in tissue optical clearing for spectroscopic application

AY Sdobnov, ME Darvin, EA Genina… - … Acta Part A: Molecular …, 2018 - Elsevier
This paper aims to review recent progress in optical clearing of the skin and over naturally
turbid biological tissues and blood using this technique in vivo and in vitro with multiphoton …