Advances in non-coding RNA sequencing

J Micheel, A Safrastyan, D Wollny - Non-coding RNA, 2021 - mdpi.com
Non-coding RNAs (ncRNAs) comprise a set of abundant and functionally diverse RNA
molecules. Since the discovery of the first ncRNA in the 1960s, ncRNAs have been shown to …

Efficient gene knockout in goats using CRISPR/Cas9 system

W Ni, J Qiao, S Hu, X Zhao, M Regouski, M Yang… - PloS one, 2014 - journals.plos.org
The CRISPR/Cas9 system has been adapted as an efficient genome editing tool in
laboratory animals such as mice, rats, zebrafish and pigs. Here, we report that …

Production of recombinant proteins in the milk of transgenic animals: current state and prospects

MV Shepelev, SV Kalinichenko, AV Deykin… - Acta Naturae …, 2018 - cyberleninka.ru
The use of transgenic animals as bioreactors for the synthesis of the recombinant proteins
secreted into milk is a current trend in the development of biotechnologies. Advances in …

Generation of mastitis resistance in cows by targeting human lysozyme gene to β-casein locus using zinc-finger nucleases

X Liu, Y Wang, Y Tian, Y Yu, M Gao… - … of the Royal …, 2014 - royalsocietypublishing.org
Mastitis costs the dairy industry billions of dollars annually and is the most consequential
disease of dairy cattle. Transgenic cows secreting an antimicrobial peptide demonstrated …

[HTML][HTML] Designing a transgenic chicken: applying new approaches toward a promising bioreactor

S Bahrami, A Amiri-Yekta, A Daneshipour… - Cell Journal …, 2020 - ncbi.nlm.nih.gov
Specific developmental characteristics of the chicken make it an attractive model for the
generation of transgenic organisms. Chicken possess a strong potential for recombinant …

Different cryopreservation requirements in foetal versus adult skin cells from an endangered mammal, the Iberian lynx (Lynx pardinus)

T León-Quinto, MA Simón, R Cadenas, Á Martínez… - Cryobiology, 2014 - Elsevier
Cryobanking somatic foetal cells acquire much relevance in endangered species for
biodiversity conservation purposes. Such cells could be later used to reintroduce the lost …

Contrasting effects of G1. 2/G2. 2 and SOF1/SOF2 embryo culture media on pre-and post-implantation development of non-transgenic and transgenic cloned goat …

SM Hosseini, M Hajian, S Ostadhosseini… - Reproductive …, 2015 - Elsevier
This study compared the efficiency of two embryo culture media (SOF1/SOF2 and G1. 2/G2.
2) for pre-and post-implantation development of somatic cell nuclear transfer goat embryos …

Optimal number of embryos for transplantation in obtaining genetic-modified mice and goats

YY Silaeva, YK Kirikovich, LN Skuratovskaya… - Russian Journal of …, 2018 - Springer
The technology of creating genetically modified animals (placental mammals) by
microinjection into the pronucleus of a fertilized egg suggests, as one of the key stages, the …

Developmental Outcome and Related Abnormalities in Goats: Comparison Between Somatic Cell Nuclear Transfer- and In Vivo-Derived Concepti During Pregnancy …

LT Martins, SG Neto, KCS Tavares… - … " Cloning and Stem …, 2016 - liebertpub.com
Cloning by somatic cell nuclear transfer (SCNT) is characterized by low efficiency and the
occurrence of developmental abnormalities, which are rather poorly studied phenomena in …

Cytokine gene expression at the maternal–fetal interface after somatic cell nuclear transfer pregnancies in small ruminants

HM Rutigliano, A Wilhelm, J Hall, B Shi… - Reproduction …, 2017 - CSIRO Publishing
The present retrospective study investigated pregnancy rates, the incidence of pregnancy
loss and large offspring syndrome (LOS) and immune-related gene expression of sheep and …