Properties of the amniotic membrane for potential use in tissue engineering
An important component of tissue engineering (TE) is the supporting matrix upon which cells
and tissues grow, also known as the scaffold. Scaffolds must easily integrate with host tissue …
and tissues grow, also known as the scaffold. Scaffolds must easily integrate with host tissue …
Derive and conquer: sourcing and differentiating stem cells for therapeutic applications
I Klimanskaya, N Rosenthal, R Lanza - Nature reviews Drug discovery, 2008 - nature.com
Although great progress has been made in the isolation and culture of stem cells, the future
of stem-cell-based therapies and their productive use in drug discovery and regenerative …
of stem-cell-based therapies and their productive use in drug discovery and regenerative …
Albumin-associated lipids regulate human embryonic stem cell self-renewal
FR Garcia-Gonzalo, JC Izpisua Belmonte - PloS one, 2008 - journals.plos.org
Background Although human embryonic stem cells (hESCs) hold great promise as a source
of differentiated cells to treat several human diseases, many obstacles still need to be …
of differentiated cells to treat several human diseases, many obstacles still need to be …
Efficient differentiation of human embryonic stem cells to arterial and venous endothelial cells under feeder-and serum-free conditions
Background Heterogeneity of endothelial cells (ECs) is a hallmark of the vascular system
which may impact the development and management of vascular disorders. Despite the …
which may impact the development and management of vascular disorders. Despite the …
Use of a synthetic xeno-free culture substrate for induced pluripotent stem cell induction and retinal differentiation
BA Tucker, KR Anfinson, RF Mullins… - Stem cells …, 2013 - academic.oup.com
The purpose of this study was to determine whether a proprietary xeno-free synthetic culture
surface could be used to aid in the production and subsequent retinal-specific differentiation …
surface could be used to aid in the production and subsequent retinal-specific differentiation …
Expression profiles of miRNAs in human embryonic stem cells during hepatocyte differentiation
Aim: Human embryonic stem cells (hESCs) are able to self‐renew and differentiate into a
variety of cell types. Although miRNAs have emerged as key regulators in the cellular …
variety of cell types. Although miRNAs have emerged as key regulators in the cellular …
Design of polymeric materials for culturing human pluripotent stem cells: Progress toward feeder-free and xeno-free culturing
A Higuchi, QD Ling, SS Kumar, M Munusamy… - Progress in Polymer …, 2014 - Elsevier
This review describes recent developments regarding the use of natural and synthetic
polymers to support the propagation of human pluripotent stem cells (hPSCs), human …
polymers to support the propagation of human pluripotent stem cells (hPSCs), human …
Derivation and propagation of human embryonic stem cell lines from frozen embryos in an animal product–free environment
E Stephenson, L Jacquet, C Miere, V Wood… - Nature protocols, 2012 - nature.com
The protocols described here are comprehensive instructions for deriving human embryonic
stem (hES) cell lines in xeno-free conditions from cryopreserved embryos. Details are …
stem (hES) cell lines in xeno-free conditions from cryopreserved embryos. Details are …
Expanding the boundaries of embryonic stem cells
U Ben-David, O Kopper, N Benvenisty - Cell Stem Cell, 2012 - cell.com
The boundaries of embryonic stem cell (ESC) research have extended considerably in
recent years in several important ways. Alongside a deeper understanding of the pluripotent …
recent years in several important ways. Alongside a deeper understanding of the pluripotent …
Isolation and propagation of mouse embryonic fibroblasts and preparation of mouse embryonic feeder layer cells
A E. Michalska - Current protocols in stem cell biology, 2007 - Wiley Online Library
To realize their potentials, embryonic stem (ES) cells must be maintained in optimal culture
conditions that preserve their pluripotency and self‐renewal capacity. Mouse embryonic …
conditions that preserve their pluripotency and self‐renewal capacity. Mouse embryonic …