The bacterial two-hybrid system based on the reconstitution of adenylate cyclase in
Escherichia coli (BACTH) was described 14years ago (Karimova, Pidoux, Ullmann, and …

The Type VI secretion system (T6SS) delivers protein effectors to diverse cell types including
prokaryotic and eukaryotic cells, therefore it participates in inter-bacterial competition and …

The yeast two-hybrid system pioneered the field of in vivo protein-protein interaction
methods and undisputedly gave rise to a palette of ingenious techniques that are constantly …

Background Clostridium difficile strain 630Δ erm is a spontaneous erythromycin sensitive
derivative of the reference strain 630 obtained by serial passaging in antibiotic-free media. It …

Wolbachia (Anaplasmataceae) is an endosymbiont of arthropods and nematodes that
resides within host cells and is well known for manipulating host biology to facilitate …

One of the leading causes of morbidity and mortality in cystic fibrosis (CF) patients is
pulmonary infection with Pseudomonas aeruginosa, and the pathophysiology of pulmonary …

The bacterial adenylate cyclase two‐hybrid (BACTH) system has been widely used to
characterize protein–protein interactions in the prokaryotic world. This system relies on the …

Bacterial gene regulation is controlled by complex regulatory cascades which integrate input
environmental signals and adapt specific and adequate output cellular responses. These …

IglE is a small, hypothetical protein encoded by the duplicated Francisella pathogenicity
island (FPI). Inactivation of both copies of iglE rendered Francisella tularensis subsp …

FipB, an essential virulence factor of Francisella tularensis, is a lipoprotein with two
conserved domains that have similarity to disulfide bond formation A (DsbA) proteins and …